Role of Pre-rRNA Base Pairing and 80S Complex Formation in Subnucleolar Localization of the U3 SnoRNP

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2004 Sep 16

PMID 15367679

Citations 30

Authors

Sander Granneman

Judith Vogelzangs

Reinhard Luhrmann

Walther J van Venrooij

Ger J M Pruijn

Nicholas J Watkins

Affiliations

Soon will be listed here.

Abstract

In the nucleolus the U3 snoRNA is recruited to the 80S pre-rRNA processing complex in the dense fibrillar component (DFC). The U3 snoRNA is found throughout the nucleolus and has been proposed to move with the preribosomes to the granular component (GC). In contrast, the localization of other RNAs, such as the U8 snoRNA, is restricted to the DFC. Here we show that the incorporation of the U3 snoRNA into the 80S processing complex is not dependent on pre-rRNA base pairing sequences but requires the B/C motif, a U3-specific protein-binding element. We also show that the binding of Mpp10 to the 80S U3 complex is dependent on sequences within the U3 snoRNA that base pair with the pre-rRNA adjacent to the initial cleavage site. Furthermore, mutations that inhibit 80S complex formation and/or the association of Mpp10 result in retention of the U3 snoRNA in the DFC. From this we propose that the GC localization of the U3 snoRNA is a direct result of its active involvement in the initial steps of ribosome biogenesis.

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