Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2020 May 10

PMID 32384686

Citations 10

Authors

Francisco Guillen-Chable

Ulises Rodriguez Corona

Alejandro Pereira-Santana

Andrea Bayona

Luis Carlos Rodriguez-Zapata

Cecilia Aquino

Lenka Sebestova

Nicolas Vitale

Pavel Hozak

Enrique Castano

Affiliations

Soon will be listed here.

Abstract

Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli.

Citing Articles

Advances in the structure and function of the nucleolar protein fibrillarin.

Zhang X, Li W, Sun S, Liu Y Front Cell Dev Biol. 2024; 12:1494631.

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Phosphorylation of Orc6 During Mitosis Regulates DNA Replication and Ribosome Biogenesis.

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Functional Tuning of Intrinsically Disordered Regions in Human Proteins by Composition Bias.

Kastano K, Mier P, Dosztanyi Z, Promponas V, Andrade-Navarro M Biomolecules. 2022; 12(10).

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Phase Separation of Intrinsically Disordered Nucleolar Proteins Relate to Localization and Function.

Guillen-Chable F, Bayona A, Rodriguez-Zapata L, Castano E Int J Mol Sci. 2021; 22(23).

PMID: 34884901 PMC: 8657925. DOI: 10.3390/ijms222313095.

Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates.

Hoboth P, Sebesta O, Sztacho M, Castano E, Hozak P MethodsX. 2021; 8:101372.

PMID: 34430268 PMC: 8374474. DOI: 10.1016/j.mex.2021.101372.

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