A Rare-cell Detector for Cancer

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Jul 14

PMID 15249663

Citations 137

Authors

Robert T Krivacic

Andras Ladanyi

Douglas N Curry

H B Hsieh

Peter Kuhn

Danielle E Bergsrud

Jane F Kepros

Todd Barbera

Michael Y Ho

Lan Bo Chen

Richard A Lerner

Richard H Bruce

Affiliations

Soon will be listed here.

Abstract

Although a reliable method for detection of cancer cells in blood would be an important tool for diagnosis and monitoring of solid tumors in early stages, current technologies cannot reliably detect the extremely low concentrations of these rare cells. The preferred method of detection, automated digital microscopy (ADM), is too slow to scan the large substrate areas. Here we report an approach that uses fiber-optic array scanning technology (FAST), which applies laser-printing techniques to the rare-cell detection problem. With FAST cytometry, laser-printing optics are used to excite 300,000 cells per sec, and emission is collected in an extremely wide field of view, enabling a 500-fold speed-up over ADM with comparable sensitivity and superior specificity. The combination of FAST enrichment and ADM imaging has the performance required for reliable detection of early-stage cancer in blood.

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