Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice Under TET Control

Overview

Journal PLoS Biol

Specialty Biology

Date 2004 Jun 23

PMID 15208716

Citations 89

Authors

Mazahir T Hasan

Rainer W Friedrich

Thomas Euler

Matthew E Larkum

Gunter Giese

Matthias Both

Jens Duebel

Jack Waters

Hermann Bujard

Oliver Griesbeck

Roger Y Tsien

Takeharu Nagai

Atsushi Miyawaki

Winfried Denk

Affiliations

Soon will be listed here.

Abstract

Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.

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