A Highly Discriminating Quencher-free Molecular Beacon for Probing DNA

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2004 May 27

PMID 15161261

Citations 18

Authors

Gil Tae Hwang

Young Jun Seo

Byeang Hyean Kim

Affiliations

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Abstract

We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.

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