Fluorescence Detection of Single-nucleotide Polymorphisms with a Single, Self-complementary, Triple-stem DNA Probe

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2009 May 12

PMID 19431180

Citations 27

Authors

Yi Xiao

Kory J I Plakos

Xinhui Lou

Ryan J White

Jiangrong Qian

Kevin W Plaxco

H Tom Soh

Affiliations

Soon will be listed here.

Abstract

Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F = fluorophore, Q = quencher).

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