High Expression of Interleukin-1beta in the Corneal Epithelium of MRL/lpr Mice is Under the Control of Their Genetic Background

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2004 Apr 17

PMID 15086386

Citations 5

Authors

M Okamoto

M Takagi

M Kutsuna

Y Hara

M Nishihara

M C Zhang

T Matsuda

M Sakanaka

S Okamoto

M Nose

Y Ohashi

Affiliations

Soon will be listed here.

Abstract

MRL/Mp mice bearing the Fas deletion mutant gene, lpr (MRL/lpr), spontaneously develop polyarthritis, sialoadenitis and dacryoadenitis, resembling rheumatoid arthritis (RA), and also corneal involvement such as keratopathy and scleritis, which is a major complication in RA patients. In this study, we found that the expression levels of IL-1beta and MMP-1 mRNAs in cornea were high in both MRL/lpr and MRL/Mp-+/+ strains of mice at an age younger than when they develop any inflammatory lesions. This was not true of other inbred strains, even those bearing the lpr gene, and also not of (NZB x NZW) F1 lupus mice. There was no significant difference in the expression of IL-1alpha and TGFbeta in cornea in these strains. Using crosses between MRL/lpr and C3H/HeJ-lpr/lpr (C3H/lpr) mice, at least the expression of IL-1beta was found to be under the control of the MRL genetic background, likely with a recessive mode of inheritance. Considering that IL-1beta in cornea was detected particularly in the epithelial layer, the high expression of IL-1beta in cornea is most likely involved in the genetic predisposition for corneal involvement and possibly also for arthritis in an MRL strain of mice.

Citing Articles

The expression of matrix metalloproteinases and their inhibitors in corneal fibroblasts by alarmins from necrotic corneal epithelial cells.

Iwatake A, Murakami A, Ebihara N Jpn J Ophthalmol. 2017; 62(1):92-100.

PMID: 29094325 DOI: 10.1007/s10384-017-0541-x.

Gene Therapy for Modulation of T-Cell-Mediated Immune Response Provoked by Corneal Transplantation.

Pastak M, Kleff V, Saban D, Czugala M, Steuhl K, Ergun S Hum Gene Ther. 2017; 29(4):467-479.

PMID: 28990426 PMC: 5915213. DOI: 10.1089/hum.2017.044.

Effect of Different Antibiotic Chemotherapies on Infection of Primary Human Corneal Fibroblast Cells.

Cendra M, Christodoulides M, Hossain P Front Microbiol. 2017; 8:1614.

PMID: 28878761 PMC: 5572282. DOI: 10.3389/fmicb.2017.01614.

Topical interleukin-1 receptor antagonist inhibits inflammatory cell infiltration into the cornea.

Stapleton W, Chaurasia S, Medeiros F, Mohan R, Sinha S, Wilson S Exp Eye Res. 2008; 86(5):753-7.

PMID: 18346730 PMC: 2693871. DOI: 10.1016/j.exer.2008.02.001.

Clinical efficacy of buprenorphine to minimize distress in MRL/lpr mice.

Swenson J, Olgun S, Radjavi A, Kaur T, Reilly C Eur J Pharmacol. 2007; 567(1-2):67-76.

PMID: 17490635 PMC: 2570058. DOI: 10.1016/j.ejphar.2007.03.048.

References

Nakatsuru S, Terada M, Nishihara M, Kamogawa J, Miyazaki T, Qu W . Genetic dissection of the complex pathological manifestations of collagen disease in MRL/lpr mice. Pathol Int. 1999; 49(11):974-82. DOI: 10.1046/j.1440-1827.1999.00979.x. View

Stern M, Beuerman R, Fox R, Gao J, Mircheff A, Pflugfelder S . The pathology of dry eye: the interaction between the ocular surface and lacrimal glands. Cornea. 1998; 17(6):584-9. DOI: 10.1097/00003226-199811000-00002. View

Watson P, Hayreh S . Scleritis and episcleritis. Br J Ophthalmol. 1976; 60(3):163-91. PMC: 1042706. DOI: 10.1136/bjo.60.3.163. View

McGavin D, Williamson J, Forrester J, Foulds W, BUCHANAN W, Dick W . Episcleritis and scleritis. A study of their clinical manifestations and association with rheumatoid arthritis. Br J Ophthalmol. 1976; 60(3):192-226. PMC: 1042707. DOI: 10.1136/bjo.60.3.192. View

Jayson M, Easty D . Ulceration of the cornea in rheumatoid arthritis. Ann Rheum Dis. 1977; 36(5):428-32. PMC: 1000134. DOI: 10.1136/ard.36.5.428. View

Andrews B, Eisenberg R, Theofilopoulos A, Izui S, Wilson C, McCONAHEY P . Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978; 148(5):1198-215. PMC: 2185049. DOI: 10.1084/jem.148.5.1198. View

Pfister R, MURPHY G . Corneal ulceration and perforation associated with Sjögren's syndrome. Arch Ophthalmol. 1980; 98(1):89-94. DOI: 10.1001/archopht.1980.01020030091006. View

. Regulation of stromal cell collagenase production in adult rabbit cornea: in vitro stimulation and inhibition by epithelial cell products. Proc Natl Acad Sci U S A. 1980; 77(9):5331-5. PMC: 350052. DOI: 10.1073/pnas.77.9.5331. View

Mizel S, Dayer J, Krane S, Mergenhagen S . Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1). Proc Natl Acad Sci U S A. 1981; 78(4):2474-7. PMC: 319369. DOI: 10.1073/pnas.78.4.2474. View

10.

Postlethwaite A, Lachman L, Mainardi C, Kang A . Interleukin 1 stimulation of collagenase production by cultured fibroblasts. J Exp Med. 1983; 157(2):801-6. PMC: 2186943. DOI: 10.1084/jem.157.2.801. View

11.

Hoffman R, Yang H, Waggie K, Durham J, Burge J, Walker S . Band keratopathy in MRL/l and MRL/n mice. Arthritis Rheum. 1983; 26(5):645-52. DOI: 10.1002/art.1780260511. View

12.

Gowen M, Wood D, Ihrie E, Meats J, Russell R . Stimulation by human interleukin 1 of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblasts in vitro. Biochim Biophys Acta. 1984; 797(2):186-93. DOI: 10.1016/0304-4165(84)90121-1. View

13.

Jabs D, Alexander E, Green W . Ocular inflammation in autoimmune MRL/Mp mice. Invest Ophthalmol Vis Sci. 1985; 26(9):1223-9. View

14.

Fine A, Goldstein R . The effect of transforming growth factor-beta on cell proliferation and collagen formation by lung fibroblasts. J Biol Chem. 1987; 262(8):3897-902. View

15.

Raghow R, Postlethwaite A, Keski-Oja J, Moses H, Kang A . Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts. J Clin Invest. 1987; 79(4):1285-8. PMC: 424335. DOI: 10.1172/JCI112950. View

16.

Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9. DOI: 10.1006/abio.1987.9999. View

17.

Buchan G, Barrett K, Turner M, Chantry D, Maini R, Feldmann M . Interleukin-1 and tumour necrosis factor mRNA expression in rheumatoid arthritis: prolonged production of IL-1 alpha. Clin Exp Immunol. 1988; 73(3):449-55. PMC: 1541753. View

18.

Overall C, Wrana J, Sodek J . Independent regulation of collagenase, 72-kDa progelatinase, and metalloendoproteinase inhibitor expression in human fibroblasts by transforming growth factor-beta. J Biol Chem. 1989; 264(3):1860-9. View

19.

MacNaul K, Chartrain N, Lark M, Tocci M, Hutchinson N . Discoordinate expression of stromelysin, collagenase, and tissue inhibitor of metalloproteinases-1 in rheumatoid human synovial fibroblasts. Synergistic effects of interleukin-1 and tumor necrosis factor-alpha on stromelysin expression. J Biol Chem. 1990; 265(28):17238-45. View

20.

Matrisian L . Metalloproteinases and their inhibitors in matrix remodeling. Trends Genet. 1990; 6(4):121-5. DOI: 10.1016/0168-9525(90)90126-q. View