Interleukin-1 and Tumour Necrosis Factor MRNA Expression in Rheumatoid Arthritis: Prolonged Production of IL-1 Alpha

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1988 Sep 1

PMID 3264773

Citations 88

Authors

G Buchan

K Barrett

M Turner

D Chantry

R N Maini

M Feldmann

Affiliations

Soon will be listed here.

Abstract

In rheumatoid arthritis there is a chronic immune and inflammatory reaction which can lead to the destruction of the diseased joint. Cytokine gene expression was studied in synovial cells using cDNA probes specific for human interleukin 1 (IL-1), -alpha and IL-1 beta, tumour necrosis factor (TNF), -alpha and TNF beta (lymphotoxin); protein molecules which induce cartilage degradation and bone resorption. In all cases studied, IL-1 mRNA was present in freshly isolated synovial cells from fluid or membrane. Compared to levels of IL-1 mRNA found in optimally activated normal blood mononuclear cells, the levels of IL-1 alpha mRNA were high in seven of the nine patients studied, whereas IL-1 beta mRNA, the dominant form in blood, was relatively lower. TNF alpha and TNF beta mRNA were also detected. Rheumatoid synovial cells, cultured without any stimulus, continued to express high levels of IL-1 alpha mRNA for up to 5 days, compared to the 24 h response of activated blood cells; IL-1 beta mRNA in culture was also prolonged. Cultures of rheumatoid joint cells produced IL-1 bioactivity, with roughly equal amounts of IL-1 alpha and beta, as assessed using neutralizing antibodies. TNF bioactivity was also detected which may be of importance as TNF induces the production of IL-1. The finding of these mediators produced in large amounts in active rheumatoid synovial cells suggests that mutually stimulatory cell interactions, mediated by these molecules, may be important in the chronic inflammation and tissue destruction in rheumatoid arthritis.

Citing Articles

Novel peptide inhibitor of human tumor necrosis factor-α has antiarthritic activity.

Sahu D, Gupta C, Yennamalli R, Sharma S, Roy S, Hasan S Sci Rep. 2024; 14(1):12935.

PMID: 38839973 PMC: 11153517. DOI: 10.1038/s41598-024-63790-6.

Cascade of immune mechanism and consequences of inflammatory disorders.

Megha K, Joseph X, Akhil V, Mohanan P Phytomedicine. 2021; 91:153712.

PMID: 34511264 PMC: 8373857. DOI: 10.1016/j.phymed.2021.153712.

55 years in the life of Clinical & Experimental Immunology.

Taams L, Perryman M Clin Exp Immunol. 2021; 205(3):275-277.

PMID: 34409613 PMC: 8374229. DOI: 10.1111/cei.13652.

Deriving a Boolean dynamics to reveal macrophage activation with in vitro temporal cytokine expression profiles.

Ramirez R, Herrera A, Ramirez J, Qian C, Melton D, Shireman P BMC Bioinformatics. 2019; 20(1):725.

PMID: 31852428 PMC: 6921543. DOI: 10.1186/s12859-019-3304-5.

Biofunctional Nanofibrous Substrate for Local TNF-Capturing as a Strategy to Control Inflammation in Arthritic Joints.

Bacelo E, Alves da Silva M, Cunha C, Faria S, Carvalho A, Reis R Nanomaterials (Basel). 2019; 9(4).

PMID: 30965588 PMC: 6523323. DOI: 10.3390/nano9040567.

References

Boyum A . Isolation of leucocytes from human blood. A two-phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agent. Scand J Clin Lab Invest Suppl. 1968; 97:9-29. View

Dayer J, Beutler B, Cerami A . Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts. J Exp Med. 1985; 162(6):2163-8. PMC: 2187983. DOI: 10.1084/jem.162.6.2163. View

White B, Bancroft F . Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. J Biol Chem. 1982; 257(15):8569-72. View

Fontana A, Hengartner H, Weber E, Fehr K, Grob P, Cohen G . Interleukin 1 activity in the synovial fluid of patients with rheumatoid arthritis. Rheumatol Int. 1982; 2(2):49-53. DOI: 10.1007/BF00541245. View

Gowen M, Wood D, Ihrie E, McGuire M, Russell R . An interleukin 1 like factor stimulates bone resorption in vitro. Nature. 1983; 306(5941):378-80. DOI: 10.1038/306378a0. View

Dayer J, Ucla C, MACH B . Induction of human interleukin 1 mRNA measured by collagenase- and prostaglandin E2-stimulating activity in rheumatoid synovial cells. Eur J Immunol. 1984; 14(10):898-901. DOI: 10.1002/eji.1830141007. View

Schlesier M, Gartner M, Peter H . Analysis of T-cell cultures and clones from a patient with classic rheumatoid arthritis--evidence for the existence of autoreactive T-cell clones in blood and synovial fluid. Rheumatol Int. 1984; 4 Suppl:1-9. DOI: 10.1007/BF00541272. View

Gubler U, Chua A, Stern A, Hellmann C, Vitek M, DeChiara T . Recombinant human interleukin 1 alpha: purification and biological characterization. J Immunol. 1986; 136(7):2492-7. View

Tartakovsky B, Kovacs E, Takacs L, Durum S . T cell clone producing an IL 1-like activity after stimulation by antigen-presenting B cells. J Immunol. 1986; 137(1):160-6. View

10.

Dinarello C, Cannon J, Wolff S, Bernheim H, Beutler B, Cerami A . Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med. 1986; 163(6):1433-50. PMC: 2188124. DOI: 10.1084/jem.163.6.1433. View

11.

Rupp E, Cameron P, Ranawat C, Schmidt J, Bayne E . Specific bioactivities of monocyte-derived interleukin 1 alpha and interleukin 1 beta are similar to each other on cultured murine thymocytes and on cultured human connective tissue cells. J Clin Invest. 1986; 78(3):836-9. PMC: 423684. DOI: 10.1172/JCI112649. View

12.

Philip R, Epstein L . Tumour necrosis factor as immunomodulator and mediator of monocyte cytotoxicity induced by itself, gamma-interferon and interleukin-1. Nature. 1986; 323(6083):86-9. DOI: 10.1038/323086a0. View

13.

Pettipher E, Higgs G, Henderson B . Interleukin 1 induces leukocyte infiltration and cartilage proteoglycan degradation in the synovial joint. Proc Natl Acad Sci U S A. 1986; 83(22):8749-53. PMC: 387009. DOI: 10.1073/pnas.83.22.8749. View

14.

Espevik T, Nissen-Meyer J . A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J Immunol Methods. 1986; 95(1):99-105. DOI: 10.1016/0022-1759(86)90322-4. View

15.

Bird T, Saklatvala J . Identification of a common class of high affinity receptors for both types of porcine interleukin-1 on connective tissue cells. Nature. 1986; 324(6094):263-6. DOI: 10.1038/324263a0. View

16.

Thomson B, Mundy G, Chambers T . Tumor necrosis factors alpha and beta induce osteoblastic cells to stimulate osteoclastic bone resorption. J Immunol. 1987; 138(3):775-9. View

17.

Garrett I, Durie B, Nedwin G, Gillespie A, Bringman T, Sabatini M . Production of lymphotoxin, a bone-resorbing cytokine, by cultured human myeloma cells. N Engl J Med. 1987; 317(9):526-32. DOI: 10.1056/NEJM198708273170902. View

18.

Acres R, Larsen A, Gillis S, Conlon P . Production of IL-1 alpha and IL-1 beta by clones of EBV transformed, human B cells. Mol Immunol. 1987; 24(5):479-85. DOI: 10.1016/0161-5890(87)90022-8. View

19.

Turner M, Londei M, Feldmann M . Human T cells from autoimmune and normal individuals can produce tumor necrosis factor. Eur J Immunol. 1987; 17(12):1807-14. DOI: 10.1002/eji.1830171220. View

20.

Buchan G, Barrett K, Fujita T, Taniguchi T, Maini R, Feldmann M . Detection of activated T cell products in the rheumatoid joint using cDNA probes to Interleukin-2 (IL-2) IL-2 receptor and IFN-gamma. Clin Exp Immunol. 1988; 71(2):295-301. PMC: 1541449. View