Control of Yeast Filamentous-form Growth by Modules in an Integrated Molecular Network

Overview

Journal Genome Res

Specialty Genetics

Date 2004 Mar 3

PMID 14993204

Citations 47

Authors

Susanne Prinz

Iliana Avila-Campillo

Christine Aldridge

Ajitha Srinivasan

Krassen Dimitrov

Andrew F Siegel

Timothy Galitski

Affiliations

Soon will be listed here.

Abstract

On solid growth media with limiting nitrogen source, diploid budding-yeast cells differentiate from the yeast form to a filamentous, adhesive, and invasive form. Genomic profiles of mRNA levels in Saccharomyces cerevisiae yeast-form and filamentous-form cells were compared. Disparate data types, including genes implicated by expression change, filamentation genes known previously through a phenotype, protein-protein interaction data, and protein-metabolite interaction data were integrated as the nodes and edges of a filamentation-network graph. Application of a network-clustering method revealed 47 clusters in the data. The correspondence of the clusters to modules is supported by significant coordinated expression change among cluster co-member genes, and the quantitative identification of collective functions controlling cell properties. The modular abstraction of the filamentation network enables the association of filamentous-form cell properties with the activation or repression of specific biological processes, and suggests hypotheses. A module-derived hypothesis was tested. It was found that the 26S proteasome regulates filamentous-form growth.

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