Location and Nucleotide Sequence of the Gene for the Proton-translocating Subunit of Wheat Chloroplast ATP Synthase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Nov 1

PMID 16593250

Citations 41

Authors

C J Howe

A D Auffret

A Doherty

C M Bowman

T A Dyer

J C Gray

Affiliations

Soon will be listed here.

Abstract

The proton-translocating subunit of wheat chloroplast ATP synthase is encoded by a chloroplast gene that has been accurately mapped and whose nucleotide sequence has been determined. The predicted sequence of 81 amino acids has been confirmed in part by determination of the sequence of the first 40 amino acids from the NH(2) terminus of the protein, and it shows 100% homology with the known amino acid sequence of the spinach protein but no more than 35% homology with the amino acid sequences of bacterial and mitochondrial proteins. The gene shows no deviation from the "universal" genetic code and is not split. A potential ribosome binding site is located 12 nucleotides upstream from the initiation codon, but sequences homologous to prokaryotic promotors and transcription terminators are not apparent.

Citing Articles

SAGA1 and SAGA2 promote starch formation around proto-pyrenoids in Arabidopsis chloroplasts.

Atkinson N, Stringer R, Mitchell S, Seung D, McCormick A Proc Natl Acad Sci U S A. 2024; 121(4):e2311013121.

PMID: 38241434 PMC: 10823261. DOI: 10.1073/pnas.2311013121.

CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family in .

Donovan S, Mao Y, Orr D, Carmo-Silva E, McCormick A Front Genome Ed. 2021; 2:605614.

PMID: 34713229 PMC: 8525408. DOI: 10.3389/fgeed.2020.605614.

Condensation of Rubisco into a proto-pyrenoid in higher plant chloroplasts.

Atkinson N, Mao Y, Chan K, McCormick A Nat Commun. 2020; 11(1):6303.

PMID: 33298923 PMC: 7726157. DOI: 10.1038/s41467-020-20132-0.

The pyrenoidal linker protein EPYC1 phase separates with hybrid Arabidopsis-Chlamydomonas Rubisco through interactions with the algal Rubisco small subunit.

Atkinson N, Velanis C, Wunder T, Clarke D, Mueller-Cajar O, McCormick A J Exp Bot. 2019; 70(19):5271-5285.

PMID: 31504763 PMC: 6793452. DOI: 10.1093/jxb/erz275.

Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis.

Atkinson N, Leitao N, Orr D, Meyer M, Carmo-Silva E, Griffiths H New Phytol. 2017; 214(2):655-667.

PMID: 28084636 PMC: 5363358. DOI: 10.1111/nph.14414.

References

SANGER F, Coulson A, Barrell B, Smith A, Roe B . Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980; 143(2):161-78. DOI: 10.1016/0022-2836(80)90196-5. View

Shine J, Dalgarno L . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974; 71(4):1342-6. PMC: 388224. DOI: 10.1073/pnas.71.4.1342. View

Kanazawa H, Mabuchi K, Kayano T, Tamura F, Futai M . Nucleotide sequence of genes coding for dicyclohexylcarbodiimide-binding protein and the alpha subunit of proton-translocating ATPase of Escherichia coli. Biochem Biophys Res Commun. 1981; 100(1):219-25. DOI: 10.1016/s0006-291x(81)80085-x. View

Zurawski G, Perrot B, Bottomley W, Whitfeld P . The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res. 1981; 9(14):3251-70. PMC: 327350. DOI: 10.1093/nar/9.14.3251. View

Friedl P, Bienhaus G, Hoppe J, Schairer H . The dicyclohexylcarbodiimide-binding protein c of ATP synthase from Escherichia coli is not sufficient to express an efficient H+ conduction. Proc Natl Acad Sci U S A. 1981; 78(11):6643-6. PMC: 349105. DOI: 10.1073/pnas.78.11.6643. View

Borst P, Grivell L . Small is beautiful--portrait of a mitochondrial genome. Nature. 1981; 290(5806):443-4. DOI: 10.1038/290443a0. View

Gay N, Walker J . The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase. Nucleic Acids Res. 1981; 9(16):3919-26. PMC: 327405. DOI: 10.1093/nar/9.16.3919. View

Gatenby A, Castleton J, Saul M . Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase. Nature. 1981; 291(5811):117-21. DOI: 10.1038/291117a0. View

Tohdoh N, Shinozaki K, Sugiura M . Sequence of a putative promoter region for the rRNA genes of tobacco chloroplast DNA. Nucleic Acids Res. 1981; 9(20):5399-406. PMC: 327527. DOI: 10.1093/nar/9.20.5399. View

10.

Tohdoh N, Sugiura M . The complete nucleotide sequence of 16S ribosomal RNA gene from tobacco chloroplasts. Gene. 1982; 17(2):213-8. DOI: 10.1016/0378-1119(82)90074-9. View

11.

Reisfeld A, Mattoo A, Edelman M . Processing of a chloroplast-translated membrane protein in vivo. Analysis of the rapidly synthesized 32 000-dalton shield protein and its precursor in Spirodela oligorrhiza. Eur J Biochem. 1982; 124(1):125-9. DOI: 10.1111/j.1432-1033.1982.tb05914.x. View

12.

BHOWN A, Bennett J, Morgan P, Mole J . Use of fluorescamine as an effective blocking reagent to reduce the background in protein sequence analyses by the Beckman automated sequencer. Anal Biochem. 1981; 112(1):158-62. DOI: 10.1016/0003-2697(81)90274-8. View

13.

Doherty A, Gray J . Synthesis of a dicyclohexylcarbodiimide-binding proteolipid by isolated pea chloroplasts. Eur J Biochem. 1980; 108(1):131-6. DOI: 10.1111/j.1432-1033.1980.tb04703.x. View

14.

Palmer J, Thompson W . Rearrangements in the chloroplast genomes of mung bean and pea. Proc Natl Acad Sci U S A. 1981; 78(9):5533-7. PMC: 348780. DOI: 10.1073/pnas.78.9.5533. View

15.

Nelson N, Eytan E, Notsani B, Sigrist H, Gitler C . Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation. Proc Natl Acad Sci U S A. 1977; 74(6):2375-8. PMC: 432174. DOI: 10.1073/pnas.74.6.2375. View

16.

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

17.

Gibson F, Downie J, Cox G, Radik J . Mu-induced polarity in the unc operon of Escherichia coli. J Bacteriol. 1978; 134(3):728-36. PMC: 222317. DOI: 10.1128/jb.134.3.728-736.1978. View

18.

Sebald W, Graf T, Lukins H . The dicyclohexylcarbodiimide-binding protein of the mitochondrial ATPase complex from Neurospora crassa and Saccharomyces cerevisiae. Identification and isolation. Eur J Biochem. 1979; 93(3):587-99. DOI: 10.1111/j.1432-1033.1979.tb12859.x. View

19.

Downie J, Senior A, Gibson F, Cox G . A fifth gene (uncE) in the operon concerned with oxidative phosphorylation in Escherichia coli. J Bacteriol. 1979; 137(2):711-8. PMC: 218347. DOI: 10.1128/jb.137.2.711-718.1979. View

20.

Hensgens L, Grivell L, Borst P, Bos J . Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex. Proc Natl Acad Sci U S A. 1979; 76(4):1663-7. PMC: 383450. DOI: 10.1073/pnas.76.4.1663. View