Coaggregation Among Nonflocculating Bacteria Isolated from Activated Sludge

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2003 Oct 9

PMID 14532062

Citations 36

Authors

Anushree Malik

Masashi Sakamoto

Shohei Hanazaki

Masamitsu Osawa

Takanori Suzuki

Masaki Tochigi

Kazuo Kakii

Affiliations

Soon will be listed here.

Abstract

Thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. Among these strains, eight showed significant (74 to 99%) coaggregation with Acinetobacter johnsonii S35 while only four strains coaggregated, to a lesser extent (43 to 65%), with Acinetobacter junii S33. The extent and pattern of coaggregation as well as the aggregate size showed good correlation with cellular characteristics of the coaggregating partners. These strains were identified by sequencing of full-length 16S rRNA genes. A. johnsonii S35 could coaggregate with strains of several genera, such as Oligotropha carboxidovorans, Microbacterium esteraromaticum, and Xanthomonas spp. The role of Acinetobacter isolates as bridging organisms in multigeneric coaggregates is indicated. This investigation revealed the role of much-neglected nonflocculating bacteria in floc formation in activated sludge.

Citing Articles

Exploring Biofilm-Related Traits and Bile Salt Efficacy as Anti-Biofilm Agents in MDR .

Aleksic Sabo V, Skoric D, Jovanovic-Santa S, Knezevic P Antibiotics (Basel). 2024; 13(9).

PMID: 39335053 PMC: 11428928. DOI: 10.3390/antibiotics13090880.

Mechanisms of microbial co-aggregation in mixed anaerobic cultures.

Doloman A, Sousa D Appl Microbiol Biotechnol. 2024; 108(1):407.

PMID: 38963458 PMC: 11224092. DOI: 10.1007/s00253-024-13246-8.

Thauera sp. for efficient nitrate removal in continuous denitrifying moving bed biofilm reactor.

Patel R, Nerurkar A Bioprocess Biosyst Eng. 2024; 47(3):429-442.

PMID: 38441647 DOI: 10.1007/s00449-024-02977-7.

Evaluation of Human Dental Plaque Lactic Acid Bacilli for Probiotic Potential and Functional Analysis in Relevance to Oral Health.

Choksket S, Sharma S, Harshvardhan , Pal V, Jain A, Patil P Indian J Microbiol. 2023; 63(4):520-532.

PMID: 38031619 PMC: 10682319. DOI: 10.1007/s12088-023-01108-2.

Anti-Bacterial Adhesion on Abiotic and Biotic Surfaces of the Exopolysaccharide from the Marine Bacillus licheniformis B3-15.

Zammuto V, Spano A, Agostino E, Macri A, De Pasquale C, Ferlazzo G Mar Drugs. 2023; 21(5).

PMID: 37233507 PMC: 10223316. DOI: 10.3390/md21050313.

References

Nazina T, Kosareva I, Davydov A, Turova T, Novikova E, Khafizov R . [Physico-chemical and microbiological characteristics of groundwater from observation boreholes of a deep radioactive liquid waste repository]. Mikrobiologiia. 2000; 69(1):105-12. View

Rickard A, Leach S, Hall L, Buswell C, High N, Handley P . Phylogenetic relationships and coaggregation ability of freshwater biofilm bacteria. Appl Environ Microbiol. 2002; 68(7):3644-50. PMC: 126804. DOI: 10.1128/AEM.68.7.3644-3650.2002. View

Vandevoorde L, Christiaens H, Verstraete W . Prevalence of coaggregation reactions among chicken lactobacilli. J Appl Bacteriol. 1992; 72(3):214-9. DOI: 10.1111/j.1365-2672.1992.tb01826.x. View

Suzuki T, Nakayama T, Kurihara T, Nishino T, Esaki N . Cold-active lipolytic activity of psychrotrophic Acinetobacter sp. strain no. 6. J Biosci Bioeng. 2005; 92(2):144-8. DOI: 10.1263/jbb.92.144. View

Saadoun I . Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel. J Basic Microbiol. 2002; 42(6):420-8. DOI: 10.1002/1521-4028(200212)42:6<420::AID-JOBM420>3.0.CO;2-W. View

Zimmermann J, Ludwig W, Schleifer K . DNA polynucleotide probes generated from representatives of the genus Acinetobacter and their application in fluorescence in situ hybridization of environmental samples. Syst Appl Microbiol. 2001; 24(2):238-44. DOI: 10.1078/0723-2020-00041. View

Kolenbrander P . Surface recognition among oral bacteria: multigeneric coaggregations and their mediators. Crit Rev Microbiol. 1989; 17(2):137-59. DOI: 10.3109/10408418909105746. View

Reid G, McGroarty J, Angotti R, Cook R . Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens. Can J Microbiol. 1988; 34(3):344-51. DOI: 10.1139/m88-063. View

Hollender J, Dreyer U, Kornberger L, Kampfer P, Dott W . Selective enrichment and characterization of a phosphorus-removing bacterial consortium from activated sludge. Appl Microbiol Biotechnol. 2002; 58(1):106-11. DOI: 10.1007/s00253-001-0847-x. View

10.

Olofsson A, Zita A, Hermansson M . Floc stability and adhesion of green-fluorescent-protein-marked bacteria to flocs in activated sludge. Microbiology (Reading). 1998; 144 ( Pt 2):519-528. DOI: 10.1099/00221287-144-2-519. View

11.

Zita A, Hermansson M . Effects of bacterial cell surface structures and hydrophobicity on attachment to activated sludge flocs. Appl Environ Microbiol. 1997; 63(3):1168-70. PMC: 168408. DOI: 10.1128/aem.63.3.1168-1170.1997. View

12.

Kolenbrander P, ANDERSEN R, Holdeman L . Coaggregation of oral Bacteroides species with other bacteria: central role in coaggregation bridges and competitions. Infect Immun. 1985; 48(3):741-6. PMC: 261248. DOI: 10.1128/iai.48.3.741-746.1985. View

13.

Tezuka Y . Cation-dependent flocculation in a Flavobacterium species predominant in activated sludge. Appl Microbiol. 1969; 17(2):222-6. PMC: 377653. DOI: 10.1128/am.17.2.222-226.1969. View

14.

Tago Y, Aida K . Exocellular mucopolysaccharide closely related to bacterial floc formation. Appl Environ Microbiol. 1977; 34(3):308-14. PMC: 242648. DOI: 10.1128/aem.34.3.308-314.1977. View

15.

van Loosdrecht M, Lyklema J, Norde W, Schraa G, Zehnder A . Electrophoretic mobility and hydrophobicity as a measured to predict the initial steps of bacterial adhesion. Appl Environ Microbiol. 1987; 53(8):1898-901. PMC: 204021. DOI: 10.1128/aem.53.8.1898-1901.1987. View

16.

Boswell C, Dick R, Eccles H, Macaskie L . Phosphate uptake and release by Acinetobacter johnsonii in continuous culture and coupling of phosphate release to heavy metal accumulation. J Ind Microbiol Biotechnol. 2001; 26(6):333-40. DOI: 10.1038/sj.jim.7000139. View

17.

McBain A, Bartolo R, Catrenich C, Charbonneau D, Ledder R, Rickard A . Microbial characterization of biofilms in domestic drains and the establishment of stable biofilm microcosms. Appl Environ Microbiol. 2003; 69(1):177-85. PMC: 152421. DOI: 10.1128/AEM.69.1.177-185.2003. View