Identification of the Gene Encoding Transcription Factor NusG of Thermus Thermophilus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1992 Dec 1

PMID 1447157

Citations 7

Authors

T Heinrich

W Schroder

V A Erdmann

R K Hartmann

Affiliations

Soon will be listed here.

Abstract

The nusG gene of Thermus thermophilus HB8 was cloned and sequenced. It is located 388 bp downstream from tufB, which is followed by the genes for ribosomal proteins L11 and L1. No equivalent to secE preceding nusG, as in Escherichia coli, could be detected. The nusG gene product was overproduced in E. coli. A rabbit antiserum raised against the purified recombinant NusG reacted exclusively with one protein band of T. thermophilus crude extracts in Western blot (immunoblot) analyses, and no cross-reaction of the antiserum with E. coli NusG was observed. Recombinant NusG and the reacting T. thermophilus wild-type protein had identical sizes on sodium dodecyl sulfate-polyacrylamide gels. T. thermophilus and E. coli NusG have 45% identical and 22.5% similar amino acids, and similarities between the two proteins are most pronounced in carboxy-terminal regions. The T. thermophilus nusG gene could not rescue a nusG-deficient E. coli mutant strain.

Citing Articles

Structural and functional studies on the overproduced L11 protein from Thermus thermophilus.

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A chimeric disposition of the elongation factor genes in Rickettsia prowazekii.

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A NusG-like protein from Thermotoga maritima binds to DNA and RNA.

Liao D, Lurz R, Dobrinski B, Dennis P J Bacteriol. 1996; 178(14):4089-98.

PMID: 8763936 PMC: 178165. DOI: 10.1128/jb.178.14.4089-4098.1996.

Control of rRNA transcription in Escherichia coli.

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PMID: 8531889 PMC: 239391. DOI: 10.1128/mr.59.4.623-645.1995.

Residues essential for the function of SecE, a membrane component of the Escherichia coli secretion apparatus, are located in a conserved cytoplasmic region.

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