Processing and Termination of 23S RRNA-5S RRNA-tRNA(Gly) Primary Transcripts in Thermus Thermophilus HB8

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Apr 1

PMID 2013580

Citations 6

Authors

R K Hartmann

H Y Toschka

V A Erdmann

Affiliations

Soon will be listed here.

Abstract

The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region is located approximately 25 nucleotides upstream of 5S rRNA and precedes a putative 23S-5S rRNA spacer antitermination box A. Cleavage at this site and 5S rRNA 5' end formation were shown to be inseparable events. Termination of transcription at the uridine cluster following the termination-associated hairpin was shown to be efficient but leaky. Subsequent to the operon, a functional promoter was detected whose -35 box coincided with the uridine-rich termination region. The promoter directed synthesis of a beta-galactosidase fusion protein in Escherichia coli.

Citing Articles

Synthesis of orthogonal transcription-translation networks.

An W, Chin J Proc Natl Acad Sci U S A. 2009; 106(21):8477-82.

PMID: 19443689 PMC: 2689021. DOI: 10.1073/pnas.0900267106.

Gel retardation analysis of E. coli M1 RNA-tRNA complexes.

Hardt W, Schlegl J, Erdmann V, Hartmann R Nucleic Acids Res. 1993; 21(15):3521-7.

PMID: 7688454 PMC: 331454. DOI: 10.1093/nar/21.15.3521.

Point mutations in the leader boxA of a plasmid-encoded Escherichia coli rrnB operon cause defective antitermination in vivo.

Heinrich T, Condon C, Pfeiffer T, Hartmann R J Bacteriol. 1995; 177(13):3793-800.

PMID: 7601845 PMC: 177098. DOI: 10.1128/jb.177.13.3793-3800.1995.

Analysis of the gene encoding the RNA subunit of ribonuclease P from T. thermophilus HB8.

Hartmann R, Erdmann V Nucleic Acids Res. 1991; 19(21):5957-64.

PMID: 1719485 PMC: 329053. DOI: 10.1093/nar/19.21.5957.

Identification of the gene encoding transcription factor NusG of Thermus thermophilus.

Heinrich T, Schroder W, Erdmann V, Hartmann R J Bacteriol. 1992; 174(23):7859-63.

PMID: 1447157 PMC: 207507. DOI: 10.1128/jb.174.23.7859-7863.1992.

References

Wolters J, Erdmann V . Compilation of 5S rRNA and 5S rRNA gene sequences. Nucleic Acids Res. 1988; 16 Suppl:r1-70. PMC: 340909. DOI: 10.1093/nar/16.suppl.r1. View

Hartmann R, Ulbrich N, Erdmann V . An unusual rRNA operon constellation: in Thermus thermophilus HB8 the 23S/5S rRNA operon is a separate entity from the 16S rRNA operon. Biochimie. 1987; 69(10):1097-104. DOI: 10.1016/0300-9084(87)90009-5. View

Sato S, Nakada Y, Kanaya S, Tanaka T . Molecular cloning and nucleotide sequence of Thermus thermophilus HB8 trpE and trpG. Biochim Biophys Acta. 1988; 950(3):303-12. DOI: 10.1016/0167-4781(88)90126-1. View

Hopfl P, Ulrich N, Hartmann R, Ludwig W, Schleifer K . Complete nucleotide sequence of a 23S ribosomal RNA gene from Thermus thermophilus HB8. Nucleic Acids Res. 1988; 16(18):9043. PMC: 338653. DOI: 10.1093/nar/16.18.9043. View

Hartmann R, Erdmann V . Thermus thermophilus 16S rRNA is transcribed from an isolated transcription unit. J Bacteriol. 1989; 171(6):2933-41. PMC: 209997. DOI: 10.1128/jb.171.6.2933-2941.1989. View

Liesack W, Stackebrandt E . Evidence for unlinked rrn operons in the Planctomycete Pirellula marina. J Bacteriol. 1989; 171(9):5025-30. PMC: 210313. DOI: 10.1128/jb.171.9.5025-5030.1989. View

Castenholz R . Thermophilic blue-green algae and the thermal environment. Bacteriol Rev. 1969; 33(4):476-504. PMC: 378340. DOI: 10.1128/br.33.4.476-504.1969. View

Feunteun J, Jordan B, Monier R . Study of the maturation of 5 s RNA precursors in Escherichia coli. J Mol Biol. 1972; 70(3):465-74. DOI: 10.1016/0022-2836(72)90553-0. View

Pace N, PATO M, McKIBBIN J, Radcliffe C . Precursors of 5 S ribosomal RNA in Bacillus subtilis. J Mol Biol. 1973; 75(4):619-31. DOI: 10.1016/0022-2836(73)90296-9. View

10.

Sogin M, Pace B, Pace N . Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis. J Biol Chem. 1977; 252(4):1350-7. View

11.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

12.

Degryse E, Glansdorff N, PIERARD A . A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol. 1978; 117(2):189-96. DOI: 10.1007/BF00402307. View

13.

Young R . Transcription termination in the Escherichia coli ribosomal RNA operon rrnC. J Biol Chem. 1979; 254(24):12725-31. View

14.

Bram R, Young R, Steitz J . The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli. Cell. 1980; 19(2):393-401. DOI: 10.1016/0092-8674(80)90513-9. View

15.

Nagahari K, Koshikawa T, Sakaguchi K . Cloning and expression of the leucine gene from Thermus thermophilus in Escherichia coli. Gene. 1980; 10(2):137-45. DOI: 10.1016/0378-1119(80)90131-6. View

16.

Duester G, Holmes W . The distal end of the ribosomal RNA operon rrnD of Escherichia coli contains a tRNA1thr gene, two 5s rRNA genes and a transcription terminator. Nucleic Acids Res. 1980; 8(17):3793-807. PMC: 324195. DOI: 10.1093/nar/8.17.3793. View

17.

Sekiya T, Mori M, Takahashi N, Nishimura S . Sequence of the distal tRNA1Asp gene and the transcription termination signal in the Escherichia coli ribosomal RNA operon rrnF(or G). Nucleic Acids Res. 1980; 8(17):3809-27. PMC: 324196. DOI: 10.1093/nar/8.17.3809. View

18.

HAWLEY D, McClure W . Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983; 11(8):2237-55. PMC: 325881. DOI: 10.1093/nar/11.8.2237. View

19.

Hanahan D . Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 166(4):557-80. DOI: 10.1016/s0022-2836(83)80284-8. View

20.

Brosius J . Plasmid vectors for the selection of promoters. Gene. 1984; 27(2):151-60. DOI: 10.1016/0378-1119(84)90136-7. View