Regulation of Cl- Channels in Normal and Cystic Fibrosis Airway Epithelial Cells by Extracellular ATP

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Mar 1

PMID 1371880

Citations 38

Authors

M J Stutts

T C Chinet

S J Mason

J M Fullton

L L Clarke

R C Boucher

Affiliations

Soon will be listed here.

Abstract

The rate of Cl- secretion by human airway epithelium is determined, in part, by apical cell membrane Cl- conductance. In cystic fibrosis airway epithelia, defective regulation of Cl- conductance decreases the capability to secrete Cl-. Here we report that extracytosolic ATP in the luminal bath of cultured human airway epithelia increased transepithelial Cl- secretion and apical membrane Cl- permeability. Single-channel studies in excised membrane patches revealed that ATP increased the open probability of outward rectifying Cl- channels. The latter effect occurs through a receptor mechanism that requires no identified soluble second messengers and is insensitive to probes of G protein function. These results demonstrate a mode of regulation of anion channels by binding ATP at the extracellular surface. Regulation of Cl- conductance by external ATP is preserved in cystic fibrosis airway epithelia.

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