Analysis of Mitotic and Meiotic Defects in Saccharomyces Cerevisiae SRS2 DNA Helicase Mutants

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1992 Sep 1

PMID 1327956

Citations 59

Authors

F Palladino

H L Klein

Affiliations

Soon will be listed here.

Abstract

The hyper-gene conversion srs2-101 mutation of the SRS2 DNA helicase gene of Saccharomyces cerevisiae has been reported to suppress the UV sensitivity of rad18 mutants. New alleles of SRS2 were recovered using this suppressor phenotype. The alleles have been characterized with respect to suppression of rad18 UV sensitivity, hyperrecombination, reduction of meiotic viability, and definition of the mutational change within the SRS2 gene. Variability in the degree of rad18 suppression and hyperrecombination were found. The alleles that showed the severest effects were found to be missense mutations within the consensus domains of the DNA helicase family of proteins. The effect of mutations in domains I (ATP-binding) and V (proposed DNA binding) are reported. Some alleles of SRS2 reduce spore viability to 50% of wild-type levels. This phenotype is not bypassed by spo13 mutation. Although the srs2 homozygous diploids strains undergo normal commitment to meiotic recombination, this event is delayed by several hours in the mutant strains and the strains appear to stall in the progression from meiosis I to meiosis II.

Citing Articles

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References

Weeda G, Van Ham R, Vermeulen W, BOOTSMA D, Van Der Eb A, Hoeijmakers J . A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome. Cell. 1990; 62(4):777-91. DOI: 10.1016/0092-8674(90)90122-u. View

Schiestl R, Prakash S, Prakash L . The SRS2 suppressor of rad6 mutations of Saccharomyces cerevisiae acts by channeling DNA lesions into the RAD52 DNA repair pathway. Genetics. 1990; 124(4):817-31. PMC: 1203974. DOI: 10.1093/genetics/124.4.817. View

Weber C, Salazar E, Stewart S, Thompson L . ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3. EMBO J. 1990; 9(5):1437-47. PMC: 551832. DOI: 10.1002/j.1460-2075.1990.tb08260.x. View

Thommes P, Hubscher U . Eukaryotic DNA replication. Enzymes and proteins acting at the fork. Eur J Biochem. 1990; 194(3):699-712. DOI: 10.1111/j.1432-1033.1990.tb19460.x. View

Abelson J . PRP5: a helicase-like protein required for mRNA splicing in yeast. Proc Natl Acad Sci U S A. 1990; 87(11):4236-40. PMC: 54083. DOI: 10.1073/pnas.87.11.4236. View

Chang T, Arenas J, Abelson J . Identification of five putative yeast RNA helicase genes. Proc Natl Acad Sci U S A. 1990; 87(4):1571-5. PMC: 53517. DOI: 10.1073/pnas.87.4.1571. View

Gorbalenya A, Koonin E, Donchenko A, Blinov V . Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 1989; 17(12):4713-30. PMC: 318027. DOI: 10.1093/nar/17.12.4713. View

Aboussekhra A, Chanet R, Zgaga Z, Cassier-Chauvat C, Heude M, Fabre F . RADH, a gene of Saccharomyces cerevisiae encoding a putative DNA helicase involved in DNA repair. Characteristics of radH mutants and sequence of the gene. Nucleic Acids Res. 1989; 17(18):7211-9. PMC: 334801. DOI: 10.1093/nar/17.18.7211. View

Linder P, Lasko P, Ashburner M, Leroy P, Nielsen P, Nishi K . Birth of the D-E-A-D box. Nature. 1989; 337(6203):121-2. DOI: 10.1038/337121a0. View

10.

Hollingsworth N, Byers B . HOP1: a yeast meiotic pairing gene. Genetics. 1989; 121(3):445-62. PMC: 1203632. DOI: 10.1093/genetics/121.3.445. View

11.

Aguilera A, Klein H . Genetic and molecular analysis of recombination events in Saccharomyces cerevisiae occurring in the presence of the hyper-recombination mutation hpr1. Genetics. 1989; 122(3):503-17. PMC: 1203725. DOI: 10.1093/genetics/122.3.503. View

12.

Menees T, Roeder G . MEI4, a yeast gene required for meiotic recombination. Genetics. 1989; 123(4):675-82. PMC: 1203880. DOI: 10.1093/genetics/123.4.675. View

13.

Montelone B, Hoekstra M, Malone R . Spontaneous mitotic recombination in yeast: the hyper-recombinational rem1 mutations are alleles of the RAD3 gene. Genetics. 1988; 119(2):289-301. PMC: 1203412. DOI: 10.1093/genetics/119.2.289. View

14.

Sung P, Higgins D, Prakash L, Prakash S . Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP. EMBO J. 1988; 7(10):3263-9. PMC: 454747. DOI: 10.1002/j.1460-2075.1988.tb03193.x. View

15.

Poll E, Benbow R . A DNA helicase from Xenopus laevis ovaries. Biochemistry. 1988; 27(24):8701-6. DOI: 10.1021/bi00424a002. View

16.

Szostak J, Rothstein R . Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A. 1981; 78(10):6354-8. PMC: 349037. DOI: 10.1073/pnas.78.10.6354. View

17.

Ito H, Fukuda Y, Murata K, Kimura A . Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983; 153(1):163-8. PMC: 217353. DOI: 10.1128/jb.153.1.163-168.1983. View

18.

Malone R, Hoekstra M . Relationships between a hyper-rec mutation (REM1) and other recombination and repair genes in yeast. Genetics. 1984; 107(1):33-48. PMC: 1202313. DOI: 10.1093/genetics/107.1.33. View

19.

Klapholz S, Esposito R . Recombination and chromosome segregation during the single division meiosis in SPO12-1 and SPO13-1 diploids. Genetics. 1980; 96(3):589-611. PMC: 1214363. DOI: 10.1093/genetics/96.3.589. View

20.

SIEGEL E . Complementation studies with the repair-deficient uvrD3, uvrE156, and recL152 mutations in Escherichia coli. Mol Gen Genet. 1981; 184(3):526-30. DOI: 10.1007/BF00352533. View