Srs2 and Pif1 As Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2021 Sep 28

PMID 34573298

Citations 5

Authors

Aviv Meir

Eric C Greene

Affiliations

Soon will be listed here.

Abstract

Helicases are enzymes that convert the chemical energy stored in ATP into mechanical work, allowing them to move along and manipulate nucleic acids. The helicase superfamily 1 (Sf1) is one of the largest subgroups of helicases and they are required for a range of cellular activities across all domains of life. Sf1 helicases can be further subdivided into two classes called the Sf1a and Sf1b helicases, which move in opposite directions on nucleic acids. The results of this movement can range from the separation of strands within duplex nucleic acids to the physical remodeling or removal of nucleoprotein complexes. Here, we describe the characteristics of the Sf1a helicase Srs2 and the Sf1b helicase Pif1, both from the model organism , focusing on the roles that they play in homologous recombination, a DNA repair pathway that is necessary for maintaining genome integrity.

Citing Articles

Separation of function mutants underline multiple roles of the Srs2 helicase/translocase in break-induced replication in .

Di Terlizzi M, Liberi G, Pellicioli A MicroPubl Biol. 2024; 2024.

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The translocation activity of Rad54 reduces crossover outcomes during homologous recombination.

Sridalla K, Woodhouse M, Hu J, Scheer J, Ferlez B, Crickard J Nucleic Acids Res. 2024; 52(12):7031-7048.

PMID: 38828785 PMC: 11229335. DOI: 10.1093/nar/gkae474.

The separation pin distinguishes the pro- and anti-recombinogenic functions of Saccharomyces cerevisiae Srs2.

Meir A, Raina V, Rivera C, Marie L, Symington L, Greene E Nat Commun. 2023; 14(1):8144.

PMID: 38065943 PMC: 10709652. DOI: 10.1038/s41467-023-43918-4.

A four-point molecular handover during Okazaki maturation.

Botto M, Borsellini A, Lamers M Nat Struct Mol Biol. 2023; 30(10):1505-1515.

PMID: 37620586 DOI: 10.1038/s41594-023-01071-y.

Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA.

Mustafi M, Kwon Y, Sung P, Greene E J Biol Chem. 2023; 299(6):104817.

PMID: 37178921 PMC: 10279920. DOI: 10.1016/j.jbc.2023.104817.

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