Two Basic-helix-loop-helix Genes (MYC-146 and GL3) from Arabidopsis Can Activate Anthocyanin Biosynthesis in a White-flowered Matthiola Incana Mutant

Overview

Journal Plant Mol Biol

Date 2003 Sep 6

PMID 12956536

Citations 38

Authors

Nicola A Ramsay

Amanda R Walker

Mark Mooney

John C Gray

Affiliations

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Abstract

Basic helix-loop-helix (bHLH) proteins, similar to mammalian Myc transcription factors, regulate the anthocyanin biosynthetic pathway in both monocots and dicots. Two Arabidopsis bHLH genes, GLABRA3 (GL3) and MYC-146, encode proteins that are similar throughout the predicted amino acid sequence to R and DELILA, which regulate anthocyanin production in maize and snapdragon, respectively. Northern blot analysis indicates that MYC-146 is most highly expressed in flower buds and flowers. Expression of a MYC-146 cDNA from the CaMV 35S promoter was unable to complement the anthocyanin deficiency in a ttg1 mutant of Arabidopsis and resulted in no obvious phenotypic change in Columbia plants. However, transient expression of GL3 and MYC-146 upon microprojectile bombardment of petals of a white-flowered mutant of Matthiola incana was able to complement anthocyanin deficiency. The lack of anthocyanin-deficient Arabidopsis mutants mapping to the locations of GL3 and MYC-146 suggests that the two bHLH proteins may be partially redundant and overlap in function.

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References

Payne C, Zhang F, Lloyd A . GL3 encodes a bHLH protein that regulates trichome development in arabidopsis through interaction with GL1 and TTG1. Genetics. 2000; 156(3):1349-62. PMC: 1461316. DOI: 10.1093/genetics/156.3.1349. View

Forkmann G . Precursors and genetic control of anthocyanin synthesis in Matthiola incana R. Br. Planta. 2014; 137(2):159-63. DOI: 10.1007/BF00387553. View

Galway M, Masucci J, Lloyd A, Walbot V, Davis R, Schiefelbein J . The TTG gene is required to specify epidermal cell fate and cell patterning in the Arabidopsis root. Dev Biol. 1994; 166(2):740-54. DOI: 10.1006/dbio.1994.1352. View

Radicella J, Turks D, CHANDLER V . Cloning and nucleotide sequence of a cDNA encoding B-Peru, a regulatory protein of the anthocyanin pathway in maize. Plant Mol Biol. 1991; 17(1):127-30. DOI: 10.1007/BF00036813. View

Koornneef M, Dellaert L, van der Veen J . EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res. 1982; 93(1):109-23. DOI: 10.1016/0027-5107(82)90129-4. View

Roth B, Goff S, Klein T, Fromm M . C1- and R-dependent expression of the maize Bz1 gene requires sequences with homology to mammalian myb and myc binding sites. Plant Cell. 1991; 3(3):317-25. PMC: 160002. DOI: 10.1105/tpc.3.3.317. View

Murre C, Bain G, van Dijk M, Engel I, Furnari B, Massari M . Structure and function of helix-loop-helix proteins. Biochim Biophys Acta. 1994; 1218(2):129-35. DOI: 10.1016/0167-4781(94)90001-9. View

Ludwig S, Bowen B, Beach L, Wessler S . A regulatory gene as a novel visible marker for maize transformation. Science. 1990; 247(4941):449-50. DOI: 10.1126/science.247.4941.449. View

Voronova A, Baltimore D . Mutations that disrupt DNA binding and dimer formation in the E47 helix-loop-helix protein map to distinct domains. Proc Natl Acad Sci U S A. 1990; 87(12):4722-6. PMC: 54189. DOI: 10.1073/pnas.87.12.4722. View

10.

Walker A, Davison P, James C, Srinivasan N, Blundell T, Esch J . The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. Plant Cell. 1999; 11(7):1337-50. PMC: 144274. DOI: 10.1105/tpc.11.7.1337. View

11.

Schaffer R, Ramsay N, Samach A, Corden S, Putterill J, Carre I . The late elongated hypocotyl mutation of Arabidopsis disrupts circadian rhythms and the photoperiodic control of flowering. Cell. 1998; 93(7):1219-29. DOI: 10.1016/s0092-8674(00)81465-8. View

12.

Lloyd A, Walbot V, Davis R . Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992; 258(5089):1773-5. DOI: 10.1126/science.1465611. View

13.

Ludwig S, Habera L, Dellaporta S, Wessler S . Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region. Proc Natl Acad Sci U S A. 1989; 86(18):7092-6. PMC: 298000. DOI: 10.1073/pnas.86.18.7092. View

14.

Borevitz J, Xia Y, Blount J, Dixon R, Lamb C . Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell. 2001; 12(12):2383-2394. PMC: 102225. DOI: 10.1105/tpc.12.12.2383. View

15.

Heller W, Forkmann G, Britsch L, Grisebach H . Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis. Planta. 2013; 165(2):284-7. DOI: 10.1007/BF00395052. View

16.

. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 2001; 126(2):485-93. PMC: 1540115. DOI: 10.1104/pp.126.2.485. View

17.

Boyce J, Coates D, Fricker M, Evans D . Genomic sequence of a calnexin homolog from Arabidopsis thaliana. Plant Physiol. 1994; 106(4):1691. PMC: 159718. DOI: 10.1104/pp.106.4.1691. View

18.

Bevan M . Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984; 12(22):8711-21. PMC: 320409. DOI: 10.1093/nar/12.22.8711. View

19.

Nesi N, Debeaujon I, Jond C, Pelletier G, Caboche M, Lepiniec L . The TT8 gene encodes a basic helix-loop-helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques. Plant Cell. 2000; 12(10):1863-78. PMC: 149125. DOI: 10.1105/tpc.12.10.1863. View

20.

Lazo G, Stein P, Ludwig R . A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology (N Y). 1991; 9(10):963-7. DOI: 10.1038/nbt1091-963. View