The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat Protein

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1999 Jul 13

PMID 10402433

Citations 391

Authors

A R Walker

P A Davison

C M James

N Srinivasan

T L Blundell

J J Esch

M D Marks

J C Gray

Affiliations

Soon will be listed here.

Abstract

The TRANSPARENT TESTA GLABRA1 (TTG1) locus regulates several developmental and biochemical pathways in Arabidopsis, including the formation of hairs on leaves, stems, and roots, and the production of seed mucilage and anthocyanin pigments. The TTG1 locus has been isolated by positional cloning, and its identity was confirmed by complementation of a ttg1 mutant. The locus encodes a protein of 341 amino acid residues with four WD40 repeats. The protein is similar to AN11, a regulator of anthocyanin biosynthesis in petunia, and more distantly related to those of the beta subunits of heterotrimeric G proteins, which suggests a role for TTG1 in signal transduction to downstream transcription factors. The 1.5-kb TTG1 transcript is present in all major organs of Arabidopsis. Sequence analysis of six mutant alleles has identified base changes producing truncations or single amino acid changes in the TTG1 protein.

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References

Bernardi G . The human genome: organization and evolutionary history. Annu Rev Genet. 1995; 29:445-76. DOI: 10.1146/annurev.ge.29.120195.002305. View

Hung C, Lin Y, Zhang M, Pollock S, Marks M, Schiefelbein J . A common position-dependent mechanism controls cell-type patterning and GLABRA2 regulation in the root and hypocotyl epidermis of Arabidopsis. Plant Physiol. 1998; 117(1):73-84. PMC: 35023. DOI: 10.1104/pp.117.1.73. View

Hebsgaard S, Korning P, TOLSTRUP N, Engelbrecht J, Rouze P, Brunak S . Splice site prediction in Arabidopsis thaliana pre-mRNA by combining local and global sequence information. Nucleic Acids Res. 1996; 24(17):3439-52. PMC: 146109. DOI: 10.1093/nar/24.17.3439. View

Bancroft I, Westphal L, Schmidt R, Dean C . PFGE-resolved RFLP analysis and long range restriction mapping of the DNA of Arabidopsis thaliana using whole YAC clones as probes. Nucleic Acids Res. 1992; 20(23):6201-7. PMC: 334505. DOI: 10.1093/nar/20.23.6201. View

Rerie W, Feldmann K, Marks M . The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis. Genes Dev. 1994; 8(12):1388-99. DOI: 10.1101/gad.8.12.1388. View

Galway M, Masucci J, Lloyd A, Walbot V, Davis R, Schiefelbein J . The TTG gene is required to specify epidermal cell fate and cell patterning in the Arabidopsis root. Dev Biol. 1994; 166(2):740-54. DOI: 10.1006/dbio.1994.1352. View

Lambright D, Sondek J, Bohm A, Skiba N, Hamm H, SIGLER P . The 2.0 A crystal structure of a heterotrimeric G protein. Nature. 1996; 379(6563):311-9. DOI: 10.1038/379311a0. View

Carle G, Olson M . An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985; 82(11):3756-60. PMC: 397866. DOI: 10.1073/pnas.82.11.3756. View

Blundell T, Sibanda B, STERNBERG M, Thornton J . Knowledge-based prediction of protein structures and the design of novel molecules. Nature. 1987; 326(6111):347-52. DOI: 10.1038/326347a0. View

10.

Koornneef M, Dellaert L, van der Veen J . EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res. 1982; 93(1):109-23. DOI: 10.1016/0027-5107(82)90129-4. View

11.

Nicholls A, Sharp K, Honig B . Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins. 1991; 11(4):281-96. DOI: 10.1002/prot.340110407. View

12.

Pearson W, Lipman D . Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988; 85(8):2444-8. PMC: 280013. DOI: 10.1073/pnas.85.8.2444. View

13.

Murzin A . Structural principles for the propeller assembly of beta-sheets: the preference for seven-fold symmetry. Proteins. 1992; 14(2):191-201. DOI: 10.1002/prot.340140206. View

14.

Grill E, Somerville C . Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet. 1991; 226(3):484-90. DOI: 10.1007/BF00260662. View

15.

WARD E, Jen G . Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA. Plant Mol Biol. 1990; 14(4):561-8. DOI: 10.1007/BF00027501. View

16.

Hagberg L, Hull R, Hull S, Falkow S, Freter R, Svanborg Eden C . Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983; 40(1):265-72. PMC: 264844. DOI: 10.1128/iai.40.1.265-272.1983. View

17.

McGonigle B, Bouhidel K, Irish V . Nuclear localization of the Arabidopsis APETALA3 and PISTILLATA homeotic gene products depends on their simultaneous expression. Genes Dev. 1996; 10(14):1812-21. DOI: 10.1101/gad.10.14.1812. View

18.

Thorlby G, Shlumukov L, Vizir I, Yang C, Mulligan B, Wilson Z . Fine-scale molecular genetic (RFLP) and physical mapping of a 8.9 cM region on the top arm of Arabidopsis chromosome 5 encompassing the male sterility gene, ms1. Plant J. 1997; 12(2):471-9. DOI: 10.1046/j.1365-313x.1997.12020471.x. View

19.

Nam H, Giraudat J, Den Boer B, Moonan F, Loos W, Hauge B . Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana. Plant Cell. 1989; 1(7):699-705. PMC: 159806. DOI: 10.1105/tpc.1.7.699. View

20.

Marks M . MOLECULAR GENETIC ANALYSIS OF TRICHOME DEVELOPMENT IN ARABIDOPSIS. Annu Rev Plant Physiol Plant Mol Biol. 1997; 48:137-163. DOI: 10.1146/annurev.arplant.48.1.137. View