Methods for the Detection of D-Amino-Acid Oxidase

Overview

Journal Biol Proced Online

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2003 May 8

PMID 12734589

Citations 1

Authors

Ryuichi Konno

Affiliations

Soon will be listed here.

Abstract

Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.

Citing Articles

Effect of on MK-801-induced psychosis in mice.

Shukla D, Maheshwari R, Patel K, Balaraman R, Sen A Indian J Pharmacol. 2019; 50(5):227-235.

PMID: 30636825 PMC: 6302693. DOI: 10.4103/ijp.IJP_74_17.

References

Konno R, Sasaki M, ASAKURA S, Fukui K, Enami J, Niwa A . D-amino-acid oxidase is not present in the mouse liver. Biochim Biophys Acta. 1997; 1335(1-2):173-81. DOI: 10.1016/s0304-4165(96)00136-5. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Konno R . Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases. Biochim Biophys Acta. 1998; 1395(2):165-70. DOI: 10.1016/s0167-4781(97)00185-1. View

Konno R, Yamamoto K, Niwa A, Yasumura Y . Presence of D-amino-acid oxidase protein in mutant mice lacking D-amino-acid oxidase activity. Int J Biochem. 1991; 23(11):1301-5. DOI: 10.1016/0020-711x(91)90231-b. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Konno R, Yasumura Y . D-amino-acid oxidase and its physiological function. Int J Biochem. 1992; 24(4):519-24. DOI: 10.1016/0020-711x(92)90322-r. View

Konno R, Yasumura Y . Brain and kidney D-amino acid oxidases are coded by a single gene in the mouse. J Neurochem. 1984; 42(2):584-6. DOI: 10.1111/j.1471-4159.1984.tb02718.x. View

Konno R, Uchiyama S, Yasumura Y . Intraspecies and interspecies variations in the substrate specificity of D-amino acid oxidase. Comp Biochem Physiol B. 1982; 71(4):735-8. DOI: 10.1016/0305-0491(82)90490-4. View

Katagiri M, Tojo H, Horiike K, Yamano T . Immunochemical relationship of D-amino acid oxidases in various tissues and animals. Comp Biochem Physiol B. 1991; 99(2):345-50. DOI: 10.1016/0305-0491(91)90053-g. View

10.

Watanabe T, Motomura Y, Suga T . A new colorimetric determination of D-amino acid oxidase and urate oxidase activity. Anal Biochem. 1978; 86(1):310-5. DOI: 10.1016/0003-2697(78)90347-0. View

11.

Sasaki M, Konno R, Nishio M, Niwa A, Yasumura Y, Enami J . A single-base-pair substitution abolishes D-amino-acid oxidase activity in the mouse. Biochim Biophys Acta. 1992; 1139(4):315-8. DOI: 10.1016/0925-4439(92)90107-x. View

12.

KREBS H . Metabolism of amino-acids: Deamination of amino-acids. Biochem J. 1935; 29(7):1620-44. PMC: 1266672. DOI: 10.1042/bj0291620. View

13.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

14.

Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9. DOI: 10.1006/abio.1987.9999. View

15.

Konno R, Yasumura Y . Mouse mutant deficient in D-amino acid oxidase activity. Genetics. 1983; 103(2):277-85. PMC: 1219979. DOI: 10.1093/genetics/103.2.277. View