DC-SIGN (CD209) Mediates Dengue Virus Infection of Human Dendritic Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2003 Apr 12

PMID 12682107

Citations 394

Authors

Boonrat Tassaneetrithep

Timothy H Burgess

Angela Granelli-Piperno

Christine Trumpfheller

Jennifer Finke

Wellington Sun

Michael A Eller

Kovit Pattanapanyasat

Suttipant Sarasombath

Deborah L Birx

Ralph M Steinman

Sarah Schlesinger

Mary A Marovich

Affiliations

Soon will be listed here.

Abstract

Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.

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