High-resolution Analysis of Paraffin-embedded and Formalin-fixed Prostate Tumors Using Comparative Genomic Hybridization to Genomic Microarrays

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 2003 Feb 25

PMID 12598311

Citations 27

Authors

Pamela L Paris

Donna G Albertson

Janneke C Alers

Armann Andaya

Peter Carroll

Jane Fridlyand

Ajay N Jain

Sherwin Kamkar

David Kowbel

Pieter-Jaap Krijtenburg

Daniel Pinkel

Fritz H Schroder

Kees J Vissers

Vivienne J E Watson

Mark F Wildhagen

Colin Collins

Herman van Dekken

Affiliations

Soon will be listed here.

Abstract

We have used prostate cancer, the most commonly diagnosed noncutaneous neoplasm among men, to investigate the feasibility of performing genomic array analyses of archival tissue. Prostate-specific antigen and a biopsy Gleason grade have not proven to be accurate in predicting clinical outcome, yet they remain the only accepted biomarkers for prostate cancer. It is likely that distinct spectra of genomic alterations underlie these phenotypic differences, and that once identified, may be used to differentiate between indolent and aggressive tumors. Array comparative genomic hybridization allows quantitative detection and mapping of copy number aberrations in tumors and subsequent associations to be made with clinical outcome. Archived tissues are needed to have patients with sufficient clinical follow-up. In this report, 20 formalin-fixed and paraffin-embedded prostate cancer samples originating from 1986 to 1996 were studied. We present a straightforward protocol and demonstrate the utility of archived tissue for array comparative genomic hybridization with a 2400 element BAC array that provides high-resolution detection of both deletions and amplifications.

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