Degenerate Oligonucleotide Primed-polymerase Chain Reaction-based Array Comparative Genomic Hybridization for Extensive Amplicon Profiling of Breast Cancers : a New Approach for the Molecular Analysis of Paraffin-embedded Cancer Tissue

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 2001 May 5

PMID 11337360

Citations 20

Authors

Y Daigo

S F Chin

K L Gorringe

L G Bobrow

B A Ponder

P D Pharoah

C Caldas

Affiliations

Soon will be listed here.

Abstract

We have developed a protocol for degenerate oligonucleotide-primed-polymerase chain reaction-based array comparative genomic hybridization (array CGH) that, when combined with a laser microdissection technique, allows the analysis of cancer cell populations isolated from routine, formalin-fixed, paraffin-embedded tissue samples. Comparison of copy number changes detected by degenerate oligonucleotide-primed-polymerase chain reaction-based array CGH to those detected by conventional array CGH or fluorescence in situ hybridization, demonstrated that amplifications can be reliably detected. Using a genomic microarray containing 57 oncogenes, we screened a total of 28 breast cancer samples and obtained a detailed amplicon profile that is the most comprehensive to date in human breast cancer. The array CGH method described here will allow the genetic analysis of paraffin-embedded human cancer materials for example in the context of clinical trials.

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