Lymphocyte Activation by Monovalent Fragments of Antibodies Reactive with Cell Surface Carbohydrates

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1976 Mar 1

PMID 1249523

Authors

B A Sela

J L Wang

G M Edelman

Affiliations

Soon will be listed here.

Abstract

Antibodies reactive with cell surface carbohydrates were isolated from normal chicken serum and were found to be mitogenic for mouse splenic lymphocytes as assayed by both blast transformation and [3H]thymidine incorporation. The Fab' fragments of these carbohydrate-binding immunoglobulins were just as mitogenic as the divalent native antibody. Moreover, succinylated Fab' fragments, which probably would not form self-associating aggregates, showed similar mitogenic properties. All of these results indicate that, at least for saccharide-specific ligands, multipoint attachment and receptor cross-linkage on the cell to which the ligand is attached may not be a stringent requirement for activation.

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