A Role for Nucleosome Assembly Protein 1 in the Nuclear Transport of Histones H2A and H2B

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2002 Nov 29

PMID 12456659

Citations 89

Authors

Nima Mosammaparast

Courtney S Ewart

Lucy F Pemberton

Affiliations

Soon will be listed here.

Abstract

Import of core histones into the nucleus is a prerequisite for their deposition onto DNA and the assembly of chromatin. Here we demonstrate that nucleosome assembly protein 1 (Nap1p), a protein previously implicated in the deposition of histones H2A and H2B, is also involved in the transport of these two histones. We demonstrate that Nap1p can bind directly to Kap114p, the primary karyopherin/importin responsible for the nuclear import of H2A and H2B. Nap1p also serves as a bridge between Kap114p and the histone nuclear localization sequence (NLS). Nap1p acts cooperatively to increase the affinity of Kap114p for these NLSs. Nuclear accumulation of histone NLS-green fluorescent protein (GFP) reporters was decreased in deltanap1 cells. Furthermore, we demonstrate that Nap1p promotes the association of the H2A and H2B NLSs specifically with the karyopherin Kap114p. Localization studies demonstrate that Nap1p is a nucleocytoplasmic shuttling protein, and genetic experiments suggest that its shuttling is important for maintaining chromatin structure in vivo. We propose a model in which Nap1p links the nuclear transport of H2A and H2B to chromatin assembly.

Citing Articles

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Chromatin balances cell redox and energy homeostasis.

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Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.

Liao C, Wang Y, Pi W, Wang C, Wu Y, Chen W Nat Commun. 2023; 14(1):5518.

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Nucleosome retention by histone chaperones and remodelers occludes pervasive DNA-protein binding.

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