Growth of Osteoblast-like Cells on Porous Hydroxyapatite Ceramics: an in Vitro Study

Overview

Journal Biomol Eng

Publisher Elsevier

Specialties Biomedical Engineering
Biotechnology
Molecular Biology

Date 2002 Aug 31

PMID 12202171

Citations 16

Authors

L Cerroni

R Filocamo

M Fabbri

C Piconi

S Caropreso

S G Condo

Affiliations

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Abstract

Blocks of two porous synthetic hydroxyapatites (HA) with porosity fraction of 30-40 and 50-60 vol%, respectively and a coralline derived porous HA were evaluated in vitro in presence of the osteogenic line MC3T3-E1 and of L929 fibroblasts. The two tested biomaterials did not affect cellular proliferation (MTT test), but the contact inhibited alkaline phosphatase activity. Porous aggregates resulted perfectly biocompatible in the tests performed, since observations performed by light microscopy did not show any cell morphological change, osteoblast presented a stellar shape and typical pseudopodes. SEM observations showed intercellular matrix containing fibers on HA-based porous aggregates.

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