Basic FGF Mediates an Immediate Response of Articular Cartilage to Mechanical Injury

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 May 30

PMID 12034879

Citations 79

Authors

Tonia Vincent

Monika Hermansson

Mark Bolton

Robin Wait

Jeremy Saklatvala

Affiliations

Soon will be listed here.

Abstract

The extracellularly regulated kinase (ERK), one of the three types of mitogen-activated kinases, was rapidly activated after cutting porcine articular cartilage either when maintained as explants or in situ. Cutting released a soluble ERK-activating factor from the cartilage, which was purified and identified by MS as basic fibroblast growth factor (bFGF). Experiments with neutralizing Abs to bFGF and an FGFR1 tyrosine kinase inhibitor showed that this growth factor was the major ERK-activating factor released after injury. Treating cartilage with the heparin-degrading enzyme heparitinase also caused release of bFGF, suggesting the presence of an extracellular store that is sequestered in the matrix and released upon damage. Basic FGF induced the synthesis of a number of chondrocyte proteins including matrix metalloproteinases 1 and 3, tissue inhibitor of metalloproteinases-1, and glycoprotein 38, which were identified by MS. The strong induction of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 suggests that bFGF could have a role in remodeling damaged tissue.

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References

Costell M, Gustafsson E, Aszodi A, Morgelin M, Bloch W, Hunziker E . Perlecan maintains the integrity of cartilage and some basement membranes. J Cell Biol. 1999; 147(5):1109-22. PMC: 2169352. DOI: 10.1083/jcb.147.5.1109. View

Yayon A, Klagsbrun M, Esko J, Leder P, Ornitz D . Cell surface, heparin-like molecules are required for binding of basic fibroblast growth factor to its high affinity receptor. Cell. 1991; 64(4):841-8. DOI: 10.1016/0092-8674(91)90512-w. View

Nugent M, Iozzo R . Fibroblast growth factor-2. Int J Biochem Cell Biol. 2000; 32(2):115-20. DOI: 10.1016/s1357-2725(99)00123-5. View

Thiery J, Jouanneau J . Fibroblast growth factor-2. Int J Biochem Cell Biol. 2000; 32(3):263-7. DOI: 10.1016/s1357-2725(99)00133-8. View

Skaper S, Kee W, Facci L, MacDonald G, Doherty P, Walsh F . The FGFR1 inhibitor PD 173074 selectively and potently antagonizes FGF-2 neurotrophic and neurotropic effects. J Neurochem. 2000; 75(4):1520-7. DOI: 10.1046/j.1471-4159.2000.0751520.x. View

Finch A, Davis W, Carter W, Saklatvala J . Analysis of mitogen-activated protein kinase pathways used by interleukin 1 in tissues in vivo: activation of hepatic c-Jun N-terminal kinases 1 and 2, and mitogen-activated protein kinase kinases 4 and 7. Biochem J. 2001; 353(Pt 2):275-81. PMC: 1221569. DOI: 10.1042/0264-6021:3530275. View

Tetlow L, Adlam D, Woolley D . Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes. Arthritis Rheum. 2001; 44(3):585-94. DOI: 10.1002/1529-0131(200103)44:3<585::AID-ANR107>3.0.CO;2-C. View

Cao W, Li F, STEINBERG R, LaVail M . Development of normal and injury-induced gene expression of aFGF, bFGF, CNTF, BDNF, GFAP and IGF-I in the rat retina. Exp Eye Res. 2001; 72(5):591-604. DOI: 10.1006/exer.2001.0990. View

Chevalier X, Conrozier T, Gehrmann M, Claudepierre P, Mathieu P, Unger S . Tissue inhibitor of metalloprotease-1 (TIMP-1) serum level may predict progression of hip osteoarthritis. Osteoarthritis Cartilage. 2001; 9(4):300-7. DOI: 10.1053/joca.2000.0389. View

10.

Bame K . Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans. Glycobiology. 2001; 11(6):91R-98R. DOI: 10.1093/glycob/11.6.91r. View

11.

Fernig D, Gallagher J . Fibroblast growth factors and their receptors: an information network controlling tissue growth, morphogenesis and repair. Prog Growth Factor Res. 1994; 5(4):353-77. DOI: 10.1016/0955-2235(94)00007-8. View

12.

Seger R, KREBS E . The MAPK signaling cascade. FASEB J. 1995; 9(9):726-35. View

13.

Sundarraj N, Fite D, Ledbetter S, Chakravarti S, Hassell J . Perlecan is a component of cartilage matrix and promotes chondrocyte attachment. J Cell Sci. 1995; 108 ( Pt 7):2663-72. DOI: 10.1242/jcs.108.7.2663. View

14.

Wilm M, Shevchenko A, Houthaeve T, Breit S, Schweigerer L, Fotsis T . Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature. 1996; 379(6564):466-9. DOI: 10.1038/379466a0. View

15.

Noonan D, Fulle A, Valente P, Cai S, Horigan E, Sasaki M . The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule. J Biol Chem. 1991; 266(34):22939-47. View

16.

Turnbull J, Fernig D, Ke Y, Wilkinson M, Gallagher J . Identification of the basic fibroblast growth factor binding sequence in fibroblast heparan sulfate. J Biol Chem. 1992; 267(15):10337-41. View

17.

Werner S, Peters K, Longaker M, Banda M, Williams L . Large induction of keratinocyte growth factor expression in the dermis during wound healing. Proc Natl Acad Sci U S A. 1992; 89(15):6896-900. PMC: 49611. DOI: 10.1073/pnas.89.15.6896. View

18.

Guerne P, Sublet A, Lotz M . Growth factor responsiveness of human articular chondrocytes: distinct profiles in primary chondrocytes, subcultured chondrocytes, and fibroblasts. J Cell Physiol. 1994; 158(3):476-84. DOI: 10.1002/jcp.1041580312. View

19.

Oursler M . Osteoclast synthesis and secretion and activation of latent transforming growth factor beta. J Bone Miner Res. 1994; 9(4):443-52. DOI: 10.1002/jbmr.5650090402. View

20.

Mason I . The ins and outs of fibroblast growth factors. Cell. 1994; 78(4):547-52. DOI: 10.1016/0092-8674(94)90520-7. View