Arteriolar and Venular Patterning in Retinas of Mice Selectively Expressing VEGF Isoforms

Overview

Journal J Clin Invest

Specialty General Medicine

Date 2002 Feb 6

PMID 11827992

Citations 172

Authors

Ingeborg Stalmans

Yin-Shan Ng

Richard Rohan

Marcus Fruttiger

Ann Bouche

Ali Yuce

Hajime Fujisawa

Bart Hermans

Moshe Shani

Sandra Jansen

Dan Hicklin

David J Anderson

Tom Gardiner

Hans-Peter Hammes

Lieve Moons

Mieke Dewerchin

Desire Collen

Peter Carmeliet

Patricia A DAmore

Affiliations

Soon will be listed here.

Abstract

The murine VEGF gene is alternatively transcribed to yield the VEGF(120), VEGF(164), and VEGF(188) isoforms, which differ in their potential to bind to heparan sulfate and neuropilin-1 and to stimulate endothelial growth. Here, their role in retinal vascular development was studied in mice selectively expressing single isoforms. VEGF(164/164) mice were normal, healthy, and had normal retinal angiogenesis. In contrast, VEGF(120/120) mice exhibited severe defects in vascular outgrowth and patterning, whereas VEGF(188/188) mice displayed normal venular outgrowth but impaired arterial development. It is noteworthy that neuropilin-1, a receptor for VEGF(164), was predominantly expressed in retinal arterioles. These findings reveal distinct roles of the various VEGF isoforms in vascular patterning and arterial development in the retina.

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