Emi1 Regulates the Anaphase-promoting Complex by a Different Mechanism Than Mad2 Proteins

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2001 Dec 26

PMID 11751633

Citations 85

Authors

J D Reimann

B E Gardner

F Margottin-Goguet

P K Jackson

Affiliations

Soon will be listed here.

Abstract

The anaphase-promoting complex/cyclosome (APC) ubiquitin ligase is activated by Cdc20 and Cdh1 and inhibited by Mad2 and the spindle assembly checkpoint complex, Mad2B, and the early mitotic inhibitor Emi1. Mad2 inhibits APC(Cdc20), whereas Mad2B preferentially inhibits APC(Cdh1). We have examined the mechanism of APC inhibition by Emi1 and find that unlike Mad2 proteins, Emi1 binds and inhibits both APC(Cdh1) and APC(Cdc20). Also unlike Mad2, Emi1 stabilizes cyclin A in the embryo and requires zinc for its APC inhibitory activity. We find that Emi1 binds the substrate-binding region of Cdc20 and prevents substrate binding to the APC, illustrating a novel mechanism of APC inhibition.

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