Assembly and Secretion of Pentameric IgM in a Fusion Between a Nonsecreting B Cell Lymphoma and an IgG-secreting Plasmacytoma

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Jul 1

PMID 115002

Citations 22

Authors

W C Raschke

E L Mather

M E Koshland

Affiliations

Soon will be listed here.

Abstract

A new immunoglobulin product has been obtained by hybridization of mouse cell lines arrested at different stages in B lymphocyte development. One line was shown to have the characteristics of an undifferentiated B cell that synthesizes monomeric IgM as a membrane receptor but does not express J chain. The second line was represent of a fully differentiated plasma cell synthesizing large amounts of IgG and J chain, but no IgM. Fusion of the two cell types yielded independent hybrid clones that secreted pentameric IgM, normally the first product of antigen-driven B cell differentiation. Analyses of the hybrid cells indicated that the IgM was expressed as a result of complementation between the synthetic capacities of the parental lines. The hybrid cells synthesized both monomeric IgM and J chain and assembled these components into a pentameric molecule with the expected stoichiometry of one J chain per five monomeric units. These findings provided further evidence that the induction of B cell differentiation includes a signal for de novo synthesis of the J chain. Moreover, the complementation achieved by this hybridization provides a system for identifying other intracellular events in B cell differentiation to IgM secretion.

Citing Articles

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