Genetic Transformation of Yeast

Overview

Journal Biotechniques

Specialties Biomedical Engineering
Biotechnology

Date 2001 Apr 21

PMID 11314265

Citations 74

Authors

R D Gietz

R A Woods

Affiliations

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Abstract

Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for transformation by spheroplasting, LiAc/ssDNA/PEG, and electroporation are compared, and possible mechanisms for transformation are discussed.

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