Serotypic Characterization of Group A Rotaviruses Associated with Children's Diarrhea in Slovakia

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2001 Mar 29

PMID 11271830

Citations 2

Authors

J Tietzova

A Petrovicova

Affiliations

Soon will be listed here.

Abstract

A total of 368 rotavirus RNA-positive (PAGE) stool samples collected continually during 1992-95 from infants and young children under five years of age hospitalized with acute gastroenteritis were serotyped using an enzyme immunoassay with VP7-specific monoclonal antibodies (ELISA with MAbs) for serotypes G1-G4. The serotype was identified in 106 stool samples (29%). Comparison of electropherotype and serotype profile in individual samples did not show any remarkable correlation. The members of three electropherotypes (A, C, K) belonged to all 4 serotypes. The representatives of two electropherotypes (E, G) and of mixed electropherotype did not react with any of the specific monoclonal antibodies used. The distribution of the serotypes was scored as 52, 13, 14 and 7.5% for G1 G4, respectively, whereas 13% of samples reacted with two or more type-specific monoclonal antibodies. The G1 serotype dominated during the period followed.

Citing Articles

Burden of rotavirus gastroenteritis in the pediatric population in Central and Eastern Europe: serotype distribution and burden of illness.

Ogilvie I, Khoury H, El Khoury A, Goetghebeur M Hum Vaccin. 2011; 7(5):523-33.

PMID: 21422818 PMC: 3166495. DOI: 10.4161/hv.7.5.14819.

G-serotypes of group A rotaviruses in Pilsen region (Czechia).

Pazdiora P, Svecova M Folia Microbiol (Praha). 2006; 51(2):133-5.

PMID: 16821723 DOI: 10.1007/BF02932168.

References

Offit P, Shaw R, Greenberg H . Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7. J Virol. 1986; 58(2):700-3. PMC: 252967. DOI: 10.1128/JVI.58.2.700-703.1986. View

Unicomb L, Bishop R . Epidemiology of rotavirus strains infecting children throughout Australia during 1986-1987. A study of serotype and RNA electropherotype. Arch Virol. 1989; 106(1-2):23-34. DOI: 10.1007/BF01311035. View

Coulson B, Unicomb L, Pitson G, Bishop R . Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987; 25(3):509-15. PMC: 265970. DOI: 10.1128/jcm.25.3.509-515.1987. View

Tietzova J, Petrovicova A, Pazdiora P . Characterization of human rotaviruses isolated from symptomatic and asymptomatic infections. Acta Virol. 1995; 39(4):211-214. View

Szucs G, Matson D, Uj M, KUKAN E, Mihaly I, Jelenik Z . Group A rotavirus G type prevalence in two regions of Hungary. Arch Virol. 1995; 140(10):1693-703. DOI: 10.1007/BF01384334. View

Hoshino Y, Kapikian A . Rotavirus vaccine development for the prevention of severe diarrhea in infants and young children. Trends Microbiol. 1994; 2(7):242-9. DOI: 10.1016/0966-842x(94)90629-7. View

Masendycz P, Unicomb L, Kirkwood C, Bishop R . Rotavirus serotypes causing severe acute diarrhea in young children in six Australian cities, 1989 to 1992. J Clin Microbiol. 1994; 32(9):2315-7. PMC: 263994. DOI: 10.1128/jcm.32.9.2315-2317.1994. View

Hoshino Y, Sereno M, Midthun K, Flores J, Kapikian A, Chanock R . Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985; 82(24):8701-4. PMC: 391504. DOI: 10.1073/pnas.82.24.8701. View

Arista S, Vizzi E, Ferraro D, Cascio A, Di Stefano R . Distribution of VP7 serotypes and VP4 genotypes among rotavirus strains recovered from Italian children with diarrhea. Arch Virol. 1997; 142(10):2065-71. DOI: 10.1007/s007050050224. View

10.

Ahmed M, URASAWA S, Taniguchi K, Urasawa T, Kobayashi N, WAKASUGI F . Analysis of human rotavirus strains prevailing in Bangladesh in relation to nationwide floods brought by the 1988 monsoon. J Clin Microbiol. 1991; 29(10):2273-9. PMC: 270312. DOI: 10.1128/jcm.29.10.2273-2279.1991. View

11.

Estes M, Cohen J . Rotavirus gene structure and function. Microbiol Rev. 1989; 53(4):410-49. PMC: 372748. DOI: 10.1128/mr.53.4.410-449.1989. View

12.

Tietzova J, Petrovicova A . Isolation of group A human rotaviruses from faecal specimens in monkey MA-104 cells. Acta Virol. 1994; 38(1):47-50. View

13.

Midthun K, Kapikian A . Rotavirus vaccines: an overview. Clin Microbiol Rev. 1996; 9(3):423-34. PMC: 172902. DOI: 10.1128/CMR.9.3.423. View

14.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

15.

Ginevskaya V, Amitina N, Eremeeva T, SHIRMAN G, Priimagi L, Drozdov S . Electropherotypes and serotypes of human rotavirus in Estonia in 1989-1992. Arch Virol. 1994; 137(1-2):199-207. DOI: 10.1007/BF01311188. View