MRNA 5'-leader Trans-splicing in the Chordates

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2001 Feb 13

PMID 11159910

Citations 62

Authors

A E Vandenberghe

T H Meedel

K E Hastings

Affiliations

Soon will be listed here.

Abstract

We report the discovery of mRNA 5'-leader trans-splicing (SL trans-splicing) in the chordates. In the ascidian protochordate Ciona intestinalis, the mRNAs of at least seven genes undergo trans-splicing of a 16-nucleotide 5'-leader apparently derived from a 46-nucleotide RNA that shares features with previously characterized splice donor SL RNAs. SL trans-splicing was known previously to occur in several protist and metazoan phyla, however, this is the first report of SL trans-splicing within the deuterostome division of the metazoa. SL trans-splicing is not known to occur in the vertebrates. However, because ascidians are primitive chordates related to vertebrate ancestors, our findings raise the possibility of ancestral SL trans-splicing in the vertebrate lineage.

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