Nucleotide Sequence and Characterization of a Novel Cefotaxime-hydrolyzing Beta-lactamase (CTX-M-10) Isolated in Spain

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2001 Feb 13

PMID 11158766

Citations 46

Authors

A Oliver

J C Perez-Diaz

T M Coque

F Baquero

R Canton

Affiliations

Soon will be listed here.

Abstract

A cefotaxime-resistant, ceftazidime-susceptible Escherichia coli isolate was obtained from a patient with sepsis in 1997, from which a beta-lactamase with a pI of 8.1 was cloned. Cephaloridine and cefotaxime relative hydrolysis rates were 167 and 81, respectively (penicillin G rate = 100), whereas ceftazidime hydrolysis was not detected. The nucleotide sequence revealed a bla gene related to that coding for CTX-M-3. Despite 21 nucleotide substitutions, only 2 determined amino acid changes (Ala27Val and Arg38Gln). The amino acid sequence identity between this enzyme, designated CTX-M-10, and the chromosomal beta-lactamase of Kluyvera ascorbata was 81%.

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References

Gniadkowski M, Schneider I, Palucha A, Jungwirth R, MIKIEWICZ B, Bauernfeind A . Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme. Antimicrob Agents Chemother. 1998; 42(4):827-32. PMC: 105550. DOI: 10.1128/AAC.42.4.827. View

Gazouli M, Tzelepi E, Sidorenko S, Tzouvelekis L . Sequence of the gene encoding a plasmid-mediated cefotaxime-hydrolyzing class A beta-lactamase (CTX-M-4): involvement of serine 237 in cephalosporin hydrolysis. Antimicrob Agents Chemother. 1998; 42(5):1259-62. PMC: 105797. DOI: 10.1128/AAC.42.5.1259. View

Chanal C, Sirot D, Romaszko J, Bret L, Sirot J . Survey of prevalence of extended spectrum beta-lactamases among enterobacteriaceae. J Antimicrob Chemother. 1996; 38(1):127-32. DOI: 10.1093/jac/38.1.127. View

Sabate M, Tarrago R, Navarro F, Miro E, Verges C, Barbe J . Cloning and sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-9) from Escherichia coli in Spain. Antimicrob Agents Chemother. 2000; 44(7):1970-3. PMC: 89994. DOI: 10.1128/AAC.44.7.1970-1973.2000. View

Spratt B, Hedge P, te Heesen S, Edelman A . Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene. 1986; 41(2-3):337-42. DOI: 10.1016/0378-1119(86)90117-4. View

Sirot D, Sirot J, Labia R, Morand A, Courvalin P, Darfeuille-Michaud A . Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel beta-lactamase. J Antimicrob Chemother. 1987; 20(3):323-34. DOI: 10.1093/jac/20.3.323. View

Brun-Buisson C, Legrand P, Philippon A, Montravers F, Ansquer M, Duval J . Transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistant Klebsiella pneumoniae. Lancet. 1987; 2(8554):302-6. DOI: 10.1016/s0140-6736(87)90891-9. View

Seoane A, Garcia Lobo J . Nucleotide sequence of a new class A beta-lactamase gene from the chromosome of Yersinia enterocolitica: implications for the evolution of class A beta-lactamases. Mol Gen Genet. 1991; 228(1-2):215-20. DOI: 10.1007/BF00282468. View

Ibuka A, Taguchi A, Ishiguro M, Fushinobu S, Ishii Y, Kamitori S . Crystal structure of the E166A mutant of extended-spectrum beta-lactamase Toho-1 at 1.8 A resolution. J Mol Biol. 1999; 285(5):2079-87. DOI: 10.1006/jmbi.1998.2432. View

10.

Gazouli M, Tzelepi E, Markogiannakis A, Legakis N, Tzouvelekis L . Two novel plasmid-mediated cefotaxime-hydrolyzing beta-lactamases (CTX-M-5 and CTX-M-6) from Salmonella typhimurium. FEMS Microbiol Lett. 1998; 165(2):289-93. DOI: 10.1111/j.1574-6968.1998.tb13159.x. View

11.

Bradford P, Yang Y, Sahm D, Grope I, Gardovska D, Storch G . CTX-M-5, a novel cefotaxime-hydrolyzing beta-lactamase from an outbreak of Salmonella typhimurium in Latvia. Antimicrob Agents Chemother. 1998; 42(8):1980-4. PMC: 105719. DOI: 10.1128/AAC.42.8.1980. View

12.

Ma L, Ishii Y, Ishiguro M, Matsuzawa H, Yamaguchi K . Cloning and sequencing of the gene encoding Toho-2, a class A beta-lactamase preferentially inhibited by tazobactam. Antimicrob Agents Chemother. 1998; 42(5):1181-6. PMC: 105770. DOI: 10.1128/AAC.42.5.1181. View

13.

Arakawa Y, Ohta M, Kido N, Mori M, Ito H, Komatsu T . Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics. Antimicrob Agents Chemother. 1989; 33(1):63-70. PMC: 171422. DOI: 10.1128/AAC.33.1.63. View

14.

Barthelemy M, Peduzzi J, Bernard H, Tancrede C, Labia R . Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum beta-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca. Biochim Biophys Acta. 1992; 1122(1):15-22. DOI: 10.1016/0167-4838(92)90121-s. View

15.

Bauernfeind A, Stemplinger I, Jungwirth R, Ernst S, Casellas J . Sequences of beta-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases. Antimicrob Agents Chemother. 1996; 40(2):509-13. PMC: 163149. DOI: 10.1128/AAC.40.2.509. View

16.

Yuan M, Aucken H, Hall L, Pitt T, Livermore D . Epidemiological typing of klebsiellae with extended-spectrum beta-lactamases from European intensive care units. J Antimicrob Chemother. 1998; 41(5):527-39. DOI: 10.1093/jac/41.5.527. View

17.

Perilli M, Franceschini N, Segatore B, Amicosante G, Oratore A, Duez C . Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus. FEMS Microbiol Lett. 1991; 67(1):79-84. DOI: 10.1016/0378-1097(91)90448-j. View

18.

Gomez-Gomez J, Blazquez J, Espinosa de los Monteros L, Baquero M, Baquero F, Martinez J . In vitro plasmid-encoded resistance to quinolones. FEMS Microbiol Lett. 1997; 154(2):271-6. DOI: 10.1111/j.1574-6968.1997.tb12655.x. View

19.

Bonnet R, Sampaio J, Labia R, De Champs C, Sirot D, Chanal C . A novel CTX-M beta-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil. Antimicrob Agents Chemother. 2000; 44(7):1936-42. PMC: 89989. DOI: 10.1128/AAC.44.7.1936-1942.2000. View

20.

Tamaki M, Nukaga M, Sawai T . Replacement of serine 237 in class A beta-lactamase of Proteus vulgaris modifies its unique substrate specificity. Biochemistry. 1994; 33(33):10200-6. DOI: 10.1021/bi00199a049. View