Cloning and Nucleotide Sequencing of the Gene Encoding the Beta-lactamase from Citrobacter Diversus

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 1991 Sep 15

PMID 1778425

Citations 32

Authors

M Perilli

N Franceschini

B Segatore

G Amicosante

A Oratore

C Duez

B Joris

J M Frere

Affiliations

Soon will be listed here.

Abstract

The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase.

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