Cysticercosis Immunodiagnosis Using 18- and 14-kilodalton Proteins from Taenia Crassiceps Cysticercus Antigens Obtained by Immunoaffinity Chromatography

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2005 Jul 8

PMID 16000432

Citations 9

Authors

Noeli Maria Espindola

Alberto Hiroshi Iha

Irene Fernandes

Osvaldo Massaiti Takayanagui

Luis Dos Ramos Machado

Jose Antonio Livramento

Antonio Augusto Mendes Maia

Jose Mauro Peralta

Adelaide Jose Vaz

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies (MAb) against Taenia crassiceps and Taenia solium cysticerci were produced and showed cross-reactivity with a 14-kDa protein from T. solium and with 18- and 14-kDa proteins from T. crassiceps. These MAbs and antibodies from cerebrospinal fluid (CSF) as well as serum samples from patients with neurocysticercosis (NC) reacted with 18- and 14-kDa T. crassiceps proteins purified by immunoaffinity chromatography using a Sepharose column coupled with MAbs (anti-excretory/secretory or anti-vesicular fluid antigens). Immunoaffinity-purified 18- and 14-kDa proteins were used in the design of a diagnostic enzyme-linked immunosorbent assay (ELISA) to detect antibodies in 23 CSF and 20 serum samples from patients with NC, showing 100% sensitivity. The test specificity was determined using 42 noninflammatory CSF samples and 70 inflammatory CSF samples from patients with other neurological disorders (OND), showing 100% and 99.1% (confidence interval, 97.3% to 100%) specificity, respectively. A false-positive CSF sample result in the OND group was from a human immunodeficiency virus-positive patient with meningoencephalitis. By using serum samples from 194 healthy individuals, the specificity was 100%. Analysis of an additional 16 serum samples from individuals with other parasitic diseases (13 with intestinal parasitosis and 3 with schistosomiasis) showed negative results. Three (10%) serum samples from patients with hydatidosis were positive in our ELISA and in ELISA with T. solium cysticerci antigens. Two of them were also positive by immunoblotting. The use of 18- and 14-kDa T. crassiceps immunoaffinity-purified proteins for detection of anti-cysticercus antibodies in CSF and/or serum samples using an ELISA system showed a good performance and high specificity for serum samples, dispensing with the use of confirmatory tests, such as immunoblotting, for checking specificity.

Citing Articles

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India).

Satyaprakash K, Khan W, Zade N, Chaudhari S, Shinde S, Kurkure N Helminthologia. 2023; 60(3):208-220.

PMID: 38152476 PMC: 10750244. DOI: 10.2478/helm-2023-0023.

A gel-free proteomic analysis of Taenia solium and Taenia crassiceps cysticerci vesicular extracts.

Verissimo da Costa G, Peralta R, Kalume D, Alves A, Peralta J Parasitol Res. 2018; 117(12):3781-3790.

PMID: 30215138 DOI: 10.1007/s00436-018-6080-7.

Significance of a common 65 kDa antigen in the experimental fasciolosis and toxoplasmosis.

Mohamed Shaapan R, Toaleb N, Abdel-Rahman E J Parasit Dis. 2015; 39(3):550-6.

PMID: 26345069 PMC: 4554586. DOI: 10.1007/s12639-013-0394-2.

Seroprevalence of toxoplasmosis, toxocariasis and cysticercosis in a rural settlement, São Paulo State, Brazil.

Prestes-Carneiro L, Rubinsky-Elefant G, Ferreira A, Araujo P, Troiani C, Zago S Pathog Glob Health. 2013; 107(2):88-95.

PMID: 23683335 PMC: 4001483. DOI: 10.1179/2047773213Y.0000000079.

Are basophils important mediators for helminth-induced Th2 immune responses? A debate.

Leon-Cabrera S, Flisser A J Biomed Biotechnol. 2012; 2012:274150.

PMID: 22500083 PMC: 3303586. DOI: 10.1155/2012/274150.

References

Singer E, Syndulko K, Schmid P, Conrad A, Tourtellotte W . Intrathecal IgG synthesis and albumin leakage are increased in subjects with HIV-1 neurologic disease. J Acquir Immune Defic Syndr (1988). 1994; 7(3):265-71. View

Restrepo B, Obregon-Henao A, Mesa M, Gil D, Ortiz B, Mejia J . Characterisation of the carbohydrate components of Taenia solium metacestode glycoprotein antigens. Int J Parasitol. 2000; 30(6):689-96. DOI: 10.1016/s0020-7519(00)00057-6. View

Hancock K, Pattabhi S, Greene R, Yushak M, Williams F, Khan A . Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol. 2003; 133(1):115-24. DOI: 10.1016/j.molbiopara.2003.10.001. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Chung J, Bahk Y, Huh S, Kang S, Kong Y, Cho S . A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. J Infect Dis. 1999; 180(4):1307-15. DOI: 10.1086/315020. View

Pardini A, Peralta R, Vaz A, Machado L, Peralta J . Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium). Clin Diagn Lab Immunol. 2002; 9(1):190-3. PMC: 119871. DOI: 10.1128/cdli.9.1.190-193.2002. View

Bueno E, Vaz A, Machado L, Livramento J, Mielle S . Specific Taenia crassiceps and Taenia solium antigenic peptides for neurocysticercosis immunodiagnosis using serum samples. J Clin Microbiol. 2000; 38(1):146-51. PMC: 86041. DOI: 10.1128/JCM.38.1.146-151.2000. View

Ishida M, Rubinsky-Elefant G, Ferreira A, Hoshino-Shimizu S, Vaz A . Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop. 2003; 89(1):73-84. DOI: 10.1016/j.actatropica.2003.09.005. View

Peralta R, Vaz A, Pardini A, Macedo H, Machado L, De Simone S . Evaluation of an antigen from Taenia crassiceps cysticercus for the serodiagnosis of neurocysticercosis. Acta Trop. 2002; 83(2):159-68. DOI: 10.1016/s0001-706x(02)00092-x. View

10.

Pinto P, Vaz A, Germano P, Nakamura P . Performance of the ELISA test for swine cysticercosis using antigens of Taenia solium and Taenia crassiceps cysticerci. Vet Parasitol. 2000; 88(1-2):127-30. DOI: 10.1016/s0304-4017(99)00201-0. View

11.

Greene R, Wilkins P, Tsang V . Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol. 1999; 99(2):257-61. DOI: 10.1016/s0166-6851(99)00004-3. View

12.

Bueno E, Vaz A, Machado L, Livramento J, Avila S, Ferreira A . Antigen-specific suppression of cultured lymphocytes from patients with neurocysticercosis. Clin Exp Immunol. 2001; 126(2):304-10. PMC: 1906206. DOI: 10.1046/j.1365-2249.2001.01579.x. View

13.

Larralde C, Sotelo J, Montoya R, Palencia G, Padilla A, Govezensky T . Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium. Arch Pathol Lab Med. 1990; 114(9):926-8. View

14.

Pardini A, Vaz A, Dos Ramos Machado L, Livramento J . Cysticercus antigens in cerebrospinal fluid samples from patients with neurocysticercosis. J Clin Microbiol. 2001; 39(9):3368-72. PMC: 88349. DOI: 10.1128/JCM.39.9.3368-3372.2001. View

15.

Bragazza L, Vaz A, Passos A, Takayanagui O, Nakamura P, Espindola N . Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos coqueiros, SP) determined by Elisa and immunoblotting using Taenia crassiceps antigens. Rev Inst Med Trop Sao Paulo. 2002; 44(1):7-12. DOI: 10.1590/s0036-46652002000100002. View

16.

Wilson M, Bryan R, Fried J, Ware D, Schantz P, Pilcher J . Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis. 1991; 164(5):1007-9. DOI: 10.1093/infdis/164.5.1007. View

17.

Kunz J, Kalinna B, Watschke V, GEYER E . Taenia crassiceps metacestode vesicular fluid antigens shared with the Taenia solium larval stage and reactive with serum antibodies from patients with neurocysticercosis. Zentralbl Bakteriol. 1989; 271(4):510-20. DOI: 10.1016/s0934-8840(89)80113-6. View

18.

Greene R, Hancock K, Wilkins P, Tsang V . Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol. 2000; 86(5):1001-7. DOI: 10.1645/0022-3395(2000)086[1001:TSMCAS]2.0.CO;2. View

19.

Coltorti E, Cammarieri G . [Hydatidosis immunodiagnosis: determination of Taenia crassiceps cysticerws antigens in hydatid and vesicular fluid]. Rev Inst Med Trop Sao Paulo. 1993; 35(2):155-62. DOI: 10.1590/s0036-46651993000200007. View

20.

Michault A, Riviere B, Fressy P, Laporte J, Bertil G, Mignard C . [Contribution of enzyme-linked immuno-electrotransfer blot assay for the diagnosis of human neurocysticercosis]. Pathol Biol (Paris). 1990; 38(2):119-25. View