Synthesis Time of Beta-galactosidase in Escherichia Coli B/r As a Function of Growth Rate

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Jul 1

PMID 1106403

Citations 31

Authors

D G Dalbow

R Young

Affiliations

Soon will be listed here.

Abstract

By analysing the kinetics of beta-galactosidase accumulation after induction, the synthesis time of beta-galactosidase in Escherichia coli B/r was found to be 75s in rapidly growing cells (1.36 and 2.1 doublings/h), and 90s in slowly growing cells (0.63 doubling/h). These values correspond to peptide-chain-elongation rates of 16 and 13 amino acids/s respectively, in agreement with previous findings, indicating that the peptide-chain growth rate is constant (presumably maximal) in fast-growing bacteria, but decreased in slowly growing bacteria [Forchhammer & Lindahl (1971) J. Mol. Biol. 55, 563-568].

Citing Articles

How Does Allocate Proteome?.

Liao C, Priyanka P, Lai Y, Rao C, Lu T ACS Synth Biol. 2024; 13(9):2718-2732.

PMID: 39120961 PMC: 11415281. DOI: 10.1021/acssynbio.3c00537.

An optimal regulation of fluxes dictates microbial growth in and out of steady state.

Chure G, Cremer J Elife. 2023; 12.

PMID: 36896805 PMC: 10110240. DOI: 10.7554/eLife.84878.

Bridging ribosomal synthesis to cell growth through the lens of kinetics.

Le L, Zhu K, Su H Biophys J. 2022; 122(3):544-553.

PMID: 36564946 PMC: 9941725. DOI: 10.1016/j.bpj.2022.12.028.

Colloidal Physics Modeling Reveals How Per-Ribosome Productivity Increases with Growth Rate in Escherichia coli.

Maheshwari A, Sunol A, Gonzalez E, Endy D, Zia R mBio. 2022; 14(1):e0286522.

PMID: 36537810 PMC: 9973364. DOI: 10.1128/mbio.02865-22.

Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation.

Sun Y, Shao X, Zhang Y, Han L, Huang J, Xie Y Cell Biosci. 2022; 12(1):147.

PMID: 36064743 PMC: 9446538. DOI: 10.1186/s13578-022-00884-6.

References

Berg A, Zabin I . IMMUNOLOGICAL STUDIES ON BETA-GALACTOSIDASE AND THIOGALACTOSIDE TRANSACETYLASE: PROTEINS OF THE LACTOSE SYSTEM IN ESCHERICHIA COLI. J Mol Biol. 1964; 10:289-94. DOI: 10.1016/s0022-2836(64)80047-4. View

Alpers D, Tomkins G . THE ORDER OF INDUCTION AND DEINDUCTION OF THE ENZYMES OF THE LACTOSE OPERON IN E. COLI. Proc Natl Acad Sci U S A. 1965; 53:797-802. PMC: 221069. DOI: 10.1073/pnas.53.4.797. View

Hirsh J, Schleif R . In vivo experiments on the mechanism of action of L-arabinose C gene activator and lactose repressor. J Mol Biol. 1973; 80(3):433-44. DOI: 10.1016/0022-2836(73)90414-2. View

Bremer H, Dalbow D . Regulatory state of ribosomal genes and physiological changes in the concentration of free ribonucleic acid polymerase in Escherichia coli. Biochem J. 1975; 150(1):9-12. PMC: 1165697. DOI: 10.1042/bj1500009. View

Dalbow D, Bremer H . Metabolic regulation of beta-galactosidase synthesis in Escherichia coli. A test for constitutive ribosome synthesis. Biochem J. 1975; 150(1):1-8. PMC: 1165696. DOI: 10.1042/bj1500001b. View

ENGBAEK F, KJELDGAARD N, MAALOE O . Chain growth rate of -galactosidase during exponential growth and amino acid starvation. J Mol Biol. 1973; 75(1):109-18. DOI: 10.1016/0022-2836(73)90532-9. View

West I, Stein W . The kinetics of induction of -galactoside permease. Biochim Biophys Acta. 1973; 308(7):161-7. DOI: 10.1016/0005-2787(73)90133-0. View

Maizels N . The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973; 70(12):3585-9. PMC: 427285. DOI: 10.1073/pnas.70.12.3585. View

Dennis P, Bremer H . Macromolecular composition during steady-state growth of Escherichia coli B-r. J Bacteriol. 1974; 119(1):270-81. PMC: 245599. DOI: 10.1128/jb.119.1.270-281.1974. View

10.

Bremer H, Yuan D . RNA chain growth-rate in Escherichia coli. J Mol Biol. 1968; 38(2):163-80. DOI: 10.1016/0022-2836(68)90404-x. View

11.

Coffman R, Norris T, Koch A . Chain elongation rate of messenger and polypeptides in slowly growing Escherichia coli. J Mol Biol. 1971; 60(1):1-19. DOI: 10.1016/0022-2836(71)90442-6. View

12.

Rose J, Mosteller R, Yanofsky C . Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970; 51(3):541-50. DOI: 10.1016/0022-2836(70)90007-0. View

13.

MILLER Jr O, Hamkalo B, Thomas Jr C . Visualization of bacterial genes in action. Science. 1970; 169(3943):392-5. DOI: 10.1126/science.169.3943.392. View

14.

Riggs A, Bourgeois S, Cohn M . The lac repressor-operator interaction. 3. Kinetic studies. J Mol Biol. 1970; 53(3):401-17. DOI: 10.1016/0022-2836(70)90074-4. View

15.

FORCHHAMMER J, Lindahl L . Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15. J Mol Biol. 1971; 55(3):563-8. DOI: 10.1016/0022-2836(71)90337-8. View

16.

Kaempfer R, MAGASANIK B . Mechanism of beta-galactosidase induction in Escherichia coli. J Mol Biol. 1967; 27(3):475-94. DOI: 10.1016/0022-2836(67)90053-8. View

17.

Cooper S, Helmstetter C . Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968; 31(3):519-40. DOI: 10.1016/0022-2836(68)90425-7. View

18.

Kepes A, Beguin S . Peptide chain initiation and growth in the induced synthesis of beta-galactosidase. Biochim Biophys Acta. 1966; 123(3):546-60. DOI: 10.1016/0005-2787(66)90222-x. View

19.

McCarthy B, Britten R . The Synthesis of Ribosomes in E. coli: I. The Incorporation of C-Uracil into the Metabolic Pool and RNA. Biophys J. 2009; 2(1):35-47. PMC: 1366387. DOI: 10.1016/s0006-3495(62)86839-8. View

20.

CRAVEN G, Steers Jr E, ANFINSEN C . PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12. J Biol Chem. 1965; 240:2468-77. View