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Regulatory State of Ribosomal Genes and Physiological Changes in the Concentration of Free Ribonucleic Acid Polymerase in Escherichia Coli

Overview
Journal Biochem J
Specialty Biochemistry
Date 1975 Jul 1
PMID 1106405
Citations 6
Authors
H Bremer
D G Dalbow
Affiliations
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Abstract

The concept of promoter efficiency is introduced as frequency of RNA chain initiation at a given promoter normalized to the intracellular concentration of free (but functional) RNA polymerase. Previous observations from this laboratory on the synthesis of ribosomes and beta-galactosidase are used to show that during a nutritional shift-up from succinate minimal to glucose-amino acids medium (3-fold increase in steady-state growth rate) the concentration of free (active) RNA polymerase decreases to one-quarter of the pre-shift value and the promoter efficiencies of the genes for ribosomal RNA and ribosomal proteins increase 9- and 6-fold respectively. This extent of control of ribosomal genes is much greater than expected on the basis of the increase in the rate of ribosome synthesis (3-fold).

Citing Articles

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Transcription in bacteria at different DNA concentrations.

Churchward G, Bremer H, Young R J Bacteriol. 1982; 150(2):572-81.

PMID: 6175615 PMC: 216403. DOI: 10.1128/jb.150.2.572-581.1982.

Gene activities for ribosomal components in Escherichia coli B/r.

Bremer H, Dennis P Biochem J. 1975; 150(3):469-75.

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Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate.

Dalbow D, Young R Biochem J. 1975; 150(1):13-20.

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Biosynthesis of RNA polymerase in Escherichia coli. IX. Growth-dependent variations in the synthesis rate, content and distribution of RNA polymerase.

Kawakami K, Saitoh T, Ishihama A Mol Gen Genet. 1979; 174(2):107-16.

PMID: 386039 DOI: 10.1007/BF00268348.

References
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Dalbow D . Synthesis of RNA polymerase in Escherichia coli B-r growing at different rates. J Mol Biol. 1973; 75(1):181-4. DOI: 10.1016/0022-2836(73)90537-8. View
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Bremer H, Berry L, Dennis P . Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates. J Mol Biol. 1973; 75(1):161-79. DOI: 10.1016/0022-2836(73)90536-6. View
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