Growth-rate-dependent Adjustment of Ribosome Function in the Fungus Mucor Racemosus

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 May 15

PMID 7316986

Citations 6

Authors

M Orlowski

Affiliations

Soon will be listed here.

Abstract

The dimorphic fungus Mucor racemosus was grown at rates between 0.043 and 0.434 doubling/h while maintained as yeasts or at rates between 0.21 and 0.50 doubling/h while maintained as hyphae by altering the composition of the growth medium or the gaseous environment of the cells. Yeasts at the higher growth rates contained many more ribosomes than did yeasts at the lower growth rates. They also had a higher percentage of ribosomes active in protein synthesis and a faster rate of polypeptide-chain elongation than did the slower-growing cells. Hyphal cells at faster growth rates also contained many more ribosomes and showed a faster rate of polypeptide-chain elongation than did slower-growing cells. However, the faster-growing cells had a substantially lower proportion of ribosomes active in protein synthesis than did the slower-growing hyphae. Pulse-chase experiments failed to provide any evidence of protein turnover, which might otherwise invalidate the values calculated for the peptide-chain elongation rates.

Citing Articles

Stored mRNA in sporangiospores of the fungus Mucor racemosus.

Linz J, Orlowski M J Bacteriol. 1982; 150(3):1138-44.

PMID: 7076616 PMC: 216334. DOI: 10.1128/jb.150.3.1138-1144.1982.

Growth-rate-dependent adjustment of ribosome function in chemostat-grown cells of the fungus Mucor racemosus.

Ross J, Orlowski M J Bacteriol. 1982; 149(2):650-3.

PMID: 6799491 PMC: 216554. DOI: 10.1128/jb.149.2.650-653.1982.

Relationship of internal cyclic AMP levels, rates of protein synthesis and mucor dimorphism.

Orlowski M, Ross J Arch Microbiol. 1981; 129(5):353-6.

PMID: 6269512 DOI: 10.1007/BF00406461.

Expression of three genes for elongation factor 1 alpha during morphogenesis of Mucor racemosus.

Linz J, Sypherd P Mol Cell Biol. 1987; 7(5):1925-32.

PMID: 3037339 PMC: 365297. DOI: 10.1128/mcb.7.5.1925-1932.1987.

Three genes for the elongation factor EF-1 alpha in Mucor racemosus.

Linz J, Katayama C, Sypherd P Mol Cell Biol. 1986; 6(2):593-600.

PMID: 2946933 PMC: 367550. DOI: 10.1128/mcb.6.2.593-600.1986.

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