Characterization of an Ampicillin-resistant Haemophilus Influenzae Type B

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1976 Jun 1

PMID 1084727

Citations 6

Authors

T C Eickhoff

J M Ehret

R D BAINES

Affiliations

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Abstract

A 28-year-old female in Denver was found in early 1974 to have frontal sinusitis, osteomyelitis, and bacteremia due to Haemophilus influenzae, type B. The minimal inhibitory concentration of ampicillin for this organism was 100 mug/ml and the minimal bactericidal concentration was >100 mug/ml. It was inhibited by chloramphenicol at 0.4 mug/ml. Further studies demonstrated that ampicillin and methicillin were synergistic against this organism. It was shown to produce a diffusible beta-lactamase. Transferase of resistance from this organism to a susceptible Haemophilus parainfluenzae and a reciprocal transfer were accomplished. A test for transformation was negative as was a test for reversal of resistance by ethylenediaminetetraacetic acid.

Citing Articles

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Influence of growth medium and supplement on growth of Haemophilus influenzae and on antibacterial activity of several antibiotics.

Bergeron M, Simard P, Provencher P J Clin Microbiol. 1987; 25(4):650-5.

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Haemophilus influenzae: antibiotic susceptibility.

Needham C Clin Microbiol Rev. 1988; 1(2):218-27.

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Growth curves, microscopic morphology, and subcultures of beta-lactamase-positive and -negative Haemophilus influenzae under the influence of ampicillin and cefamandole.

Yourassowsky E, Linden M, Lismont M Antimicrob Agents Chemother. 1979; 15(3):325-31.

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Plasmid transfer in Haemophilus influenzae.

STUY J J Bacteriol. 1979; 139(2):520-9.

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