Killer Cells: a Functional Comparison Between Natural, Immune T-cell and Antibody-dependent in Vitro Systems

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1976 Apr 1

PMID 1082915

Citations 48

Authors

R Kiessling

G Petranyi

K Karre

M Jondal

D Tracey

H Wigzell

Affiliations

Soon will be listed here.

Abstract

Previous reports have shown that spleen cells from nonimmune adult mice of certain strains do regularly kill Moloney leukemia virus-induced lymphomas in short-term 51Cr release assays. This naturally occuring killer (NK) cell had low adherent properties and had the morphological appearance of a lymphocyte. Still it lacked surface characteristics of mature T or B lymphocytes. In the present report a functional study was carried out, comparing in parallel the NK system, the T-cell killing across an H-2 barrier (anti-P815), and the antibody-dependent cell-mediated chicken red blood cell (CRBC) system. In contrast to the effector cells in the CRBC system, the NK cells were insensitive to erythrocyte antibody complement (EAC) rosette depletion and would pass through nylon wool columns. NK activity was not inhibited by the presence of heat-aggregated human or mouse gamma globulin, in contrast to the strong inhibition noted in the CRBC system. Sensitivity to trypsin pretreatment was noted in the NK system as well as in the immune P815 system, whereas the CRBC system was relatively trypsin resistant. Antitheta plus complement eliminated the anti-P815 activity, but did not touch the NK activity. The present results thus further distinguish the NK cell from cytotoxic T lymphocytes or from antibody-dependent killer cells.

Citing Articles

TCR-NK Cells: A Novel Source for Adoptive Immunotherapy of Cancer.

Karahan Z, Aras M, Sutlu T Turk J Haematol. 2023; 40(1):1-10.

PMID: 36719099 PMC: 9979742. DOI: 10.4274/tjh.galenos.2022.2022.0534.

Next Generation Immuno-Oncology Strategies: Unleashing NK Cells Activity.

Mendoza-Valderrey A, Alvarez M, De Maria A, Margolin K, Melero I, Ascierto M Cells. 2022; 11(19).

PMID: 36231109 PMC: 9562848. DOI: 10.3390/cells11193147.

The changing role of natural killer cells in cancer metastasis.

Chan I, Ewald A J Clin Invest. 2022; 132(6).

PMID: 35289318 PMC: 8920322. DOI: 10.1172/JCI143762.

Innate lymphoid cells (ILC) in SARS-CoV-2 infection.

Kumar A, Cao W, Endrias K, Kuchipudi S, Mittal S, Sambhara S Mol Aspects Med. 2021; 80:101008.

PMID: 34399986 PMC: 8361007. DOI: 10.1016/j.mam.2021.101008.

Recent advances in our understanding of the allograft response.

Hennessy C, Lewik G, Cross A, Hester J, Issa F Fac Rev. 2021; 10:21.

PMID: 33718938 PMC: 7946390. DOI: 10.12703/r/10-21.

References

Kiessling R, Klein E, Pross H, Wigzell H . "Natural" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur J Immunol. 1975; 5(2):117-21. DOI: 10.1002/eji.1830050209. View

Kiessling R, Klein E, Wigzell H . "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur J Immunol. 1975; 5(2):112-7. DOI: 10.1002/eji.1830050208. View

Hanna Jr M, Tennant R, Yuhas J, CLAPP N, Batzing B, Snodgrass M . Autogenous immunity to endogenous RNA tumor virus antigens in mice with a low natural incidence of lymphoma. Cancer Res. 1972; 32(10):2226-34. View

Greenberg A, Playfair J . Spontaneously arising cytotoxicity to the P-815-Y mastocytoma in NZB mice. Clin Exp Immunol. 1974; 16(1):99-109. PMC: 1553986. View

Cerottini J, BRUNNER K . Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974; 18:67-132. DOI: 10.1016/s0065-2776(08)60308-9. View

Nowinski R, Kaehler S . Antibody to leukemia virus: widespread occurrence in inbred mice. Science. 1974; 185(4154):869-71. DOI: 10.1126/science.185.4154.869. View

Ihle J, Yurconic Jr M, Hanna Jr M . Autogenous immunity to endogenous RNA tumor virus. Radioimmune precipitation assay of mouse serum antibody levels. J Exp Med. 1973; 138(1):194-208. PMC: 2180542. DOI: 10.1084/jem.138.1.194. View

Jondal M . Surface markers on human B and T lymphocytes. IV. Distribution of surface markers on resting and blast-transformed lymphocytes. Scand J Immunol. 1974; 3(6):739-47. DOI: 10.1111/j.1365-3083.1974.tb01309.x. View

Dickler H . Studies of the human lymphocyte receptor for heat-aggregated or antigen-complexed immunoglobulin. J Exp Med. 1974; 140(2):508-22. PMC: 2139583. DOI: 10.1084/jem.140.2.508. View

10.

Julius M, Simpson E, Herzenberg L . A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973; 3(10):645-9. DOI: 10.1002/eji.1830031011. View

11.

Cikes M, Friberg Jr S, Klein G . Progressive loss of H-2 antigens with concomitant increase of cell-surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J Natl Cancer Inst. 1973; 50(2):347-62. DOI: 10.1093/jnci/50.2.347. View

12.

Cornain S, Carnaud C, Silverman D, Klein E, Rajewsky M . Spleen-cell reactivity against transplanted neurogenic rat tumors induced by ethylnitrosourea: uncovering of tumor specificity after removal of complement-receptor-bearing lymphocytes. Int J Cancer. 1975; 16(2):301-11. DOI: 10.1002/ijc.2910160213. View

13.

Herberman R, Nunn M, LAVRIN D . Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic acid allogeneic tumors. I. Distribution of reactivity and specificity. Int J Cancer. 1975; 16(2):216-29. DOI: 10.1002/ijc.2910160204. View

14.

Oldstone M, Aoki T, Dixon F . The antibody response of mice to murine leukemia virus in spontaneous infection: absence of classical immunologic tolerance (AKR mice-complement-fixing antibodies-lymphocytic choriomeningitis virus-immunofluorescence-glomerular deposits of.... Proc Natl Acad Sci U S A. 1972; 69(1):134-8. PMC: 427561. DOI: 10.1073/pnas.69.1.134. View

15.

Komuro K, Boyse E . Induction of T lymphocytes from precursor cells in vitro by a product of the thymus. J Exp Med. 1973; 138(2):479-82. PMC: 2139400. DOI: 10.1084/jem.138.2.479. View

16.

Kedar E, Otiz de Landazuri M, Fahey J . Enzymatic enhancement of cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity. J Immunol. 1974; 112(1):26-36. View

17.

Van Boxel J, Stobo J, Paul W, Green I . Antibody-dependent lymphoid cell-mediated cytotoxicity: no requirement for thymus-derived lymphocytes. Science. 1972; 175(4018):194-6. DOI: 10.1126/science.175.4018.194. View

18.

Herberman R, Nunn M, HOLDEN H, LAVRIN D . Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. Int J Cancer. 1975; 16(2):230-9. DOI: 10.1002/ijc.2910160205. View

19.

Zarling J, Nowinski R, Bach F . Lysis of leukemia cells by spleen cells of normal mice. Proc Natl Acad Sci U S A. 1975; 72(7):2780-4. PMC: 432855. DOI: 10.1073/pnas.72.7.2780. View

20.

Aoki T, Boyse E, Old L . Occurrence of natural antibody to the G (gross) leukemia antigen in mice. Cancer Res. 1966; 26(7):1415-9. View