Mapping of in Vivo Messenger RNAs for Bacteriophage PhiX-174

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Oct 1

PMID 1068463

Citations 20

Authors

F K Fujimura

M Hayashi

Affiliations

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Abstract

In vivo messenger RNA for bacteriophage phiX174 was fractionated in agarose gels into a number of discrete species ranging in size from 0.23 X 10(6) to 2.3 X 10(6) daltons. These RNA species were eluted from the gels and hybridized to specific fragments derived from phiX-174 replicative form DNA by cleavage with restriction enzymes. A map of the orientation of in vivo messenger RNAs with respect to the bacteriophage genetic map was constructed. This map indicated that initiation of messenger RNA occurred before gene B, gene C or D, and probably before gene A and that termination occurred after genes E, F, G, and H. Termination of transcription at any particular site appeared not to be entirely effective such that a number of overlapping transcripts with the same 5'-terminus was observed. Messenger RNA for gene A seemed to be relatively unstable.

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References

Chen C, HUTCHISON 3rd C, Edgell M . Isolation and genetic localization of three phi-X174 promoter regions. Nat New Biol. 1973; 243(129):233-6. DOI: 10.1038/newbio243233a0. View

HAYASHI M, Hayashi M . Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I. J Virol. 1974; 14(5):1142-51. PMC: 355631. DOI: 10.1128/JVI.14.5.1142-1151.1974. View

Linney E, Hayashi M . Intragenic regulation of the synthesis of phi chi 174 gene A proteins. Nature. 1974; 249(455):345-8. DOI: 10.1038/249345a0. View

HAYASHI M, Hayashi M . Isolation of phi X174 specific messenger ribonucleic acids in vivo and identification of their 5' terminal nucleotides. J Virol. 1972; 9(2):207-15. PMC: 356284. DOI: 10.1128/JVI.9.2.207-215.1972. View

Vanderbilt A, Borras M, Germeraad S, Tessman I, TESSMAN E . A promoter site and polarity gradients in phage S13. Virology. 1972; 50(1):171-9. DOI: 10.1016/0042-6822(72)90357-1. View

Rosen J, Woo S, Holder J, Means A, OMalley B . Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct. Biochemistry. 1975; 14(1):69-78. DOI: 10.1021/bi00672a012. View

Clements J, SINSHEIMER R . Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells. J Virol. 1975; 15(1):151-60. PMC: 354429. DOI: 10.1128/JVI.15.1.151-160.1975. View

Smith L, SINSHEIMER R . The in vitro transcription units of bacteriophage phiX174. II. In vitro initiation sites of phiX174 transcription. J Mol Biol. 1976; 103(4):699-710. DOI: 10.1016/0022-2836(76)90204-7. View

Blin N, von Gabain A, Bujard H . Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett. 1975; 53(1):84-6. DOI: 10.1016/0014-5793(75)80688-0. View

10.

Southern E . Long range periodicities in mouse satellite DNA. J Mol Biol. 1975; 94(1):51-69. DOI: 10.1016/0022-2836(75)90404-0. View

11.

Maniatis T, Jeffrey A, van deSande H . Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975; 14(17):3787-94. DOI: 10.1021/bi00688a010. View

12.

Lee A, SINSHEIMER R . A cleavage map of bacteriophage phiX174 genome. Proc Natl Acad Sci U S A. 1974; 71(7):2882-6. PMC: 388576. DOI: 10.1073/pnas.71.7.2882. View