Gene D of Bacteriophage Phi X 174: Absence of Transcriptional and Translational Regulatory Properties

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1977 Feb 1

PMID 319253

Citations 5

Authors

T J Pollock

Affiliations

Soon will be listed here.

Abstract

The only evidence that a gene product of phi chi 174 is involved in the regulation of phage transcription was presented by Clements and Sinsheimer (1974) for gene D. I. found, in attempting to investigate this possibility further, that the D protein had no regulatory effect. When the D protein was eliminated by various nonsense mutations, all other phage proteins were synthesized at rates nearly identical to the wild-type control. There was also no significant difference in the amount of phage-specific RNA synthesis as a proportion of the total RNA synthesis when the D mutants were compared with wild type. No evidence was detected for a polar effect of gene D nonsense mutants on the expression of genes F, G, and H. The relative molar rates of synthesis of D, F, G, and H, synthesized during a normal infection were 29, 4.9, 3.3, and 1.0, respectively, with an accuracy of +/- 20%.

Citing Articles

Control of bacteriophage phi X 174 gene expression by the transcription termination factor rho in vitro.

Patrushev L, Kapitza E, Stukacheva E, Shemyakin M Mol Gen Genet. 1981; 182(3):471-6.

PMID: 6457961 DOI: 10.1007/BF00293937.

Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants.

Fassler J, Tessman I J Virol. 1981; 37(3):955-62.

PMID: 6453239 PMC: 171093. DOI: 10.1128/JVI.37.3.955-962.1981.

Gene K of bacteriophage phi X 174 codes for a nonessential protein.

TESSMAN E, Tessman I, Pollock T J Virol. 1980; 33(1):557-60.

PMID: 6445011 PMC: 288574. DOI: 10.1128/JVI.33.1.557-560.1980.

Identification of lysis protein E of bacteriophage phiX174.

Pollock T, TESSMAN E, Tessman I J Virol. 1978; 28(1):408-10.

PMID: 702655 PMC: 354281. DOI: 10.1128/JVI.28.1.408-410.1978.

Suppression of polar effects of nonsense mutations by ultraviolet irradiation.

Pollock T, TESSMAN E, Tessman I J Bacteriol. 1979; 138(1):122-5.

PMID: 438127 PMC: 218247. DOI: 10.1128/jb.138.1.122-125.1979.

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