Mutations in the J-F Intercistronic Region of Bacteriophages Phi X174 and G4 Affect the Regulation of Gene Expression

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1983 Oct 1

PMID 6224940

Citations 3

Authors

M L Romantschuk

U R Muller

Affiliations

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Abstract

The J-F intercistronic region of the genomes of bacteriophages phi X174 and G4 is transcribed but not translated. It contains the potential for a perfectly base-paired hairpin structure, which has been proposed to act as a terminator of transcription or as a regulatory region for mRNA turnover (or both). We measured phage-specific protein synthesis in mutants with modifications of the hairpin sequence and found a relative decrease in the expression of the upstream gene D as compared to the downstream genes F, G, and H in all mutants. The mutations also appeared to affect the efficiency of the gene F ribosome-binding site. These data strongly support the regulatory significance of the J-F intercistronic region and the putative hairpin structure therein.

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