Defective Thymine Dimer Excision by Cell-free Extracts of Xeroderma Pigmentosum Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Aug 1

PMID 1066689

Citations 35

Authors

K Mortelmans

E C Friedberg

H Slor

G Thomas

J E Cleaver

Affiliations

Soon will be listed here.

Abstract

Crude extracts of normal human diploid fibroblasts and of human peripheral blood lymphocytes excise thymine dimers from purified ultraviolet-irradiated DNA, or from the DNA presumably present as chromatin in unfractionated cell-free preparations of cells that had been labeled with [3H]thymidine. Extracts of xeroderma pigmentosum cells from complementation groups A, C, and D also excise thymine dimers from purified DNA, but extracts of group A cells do not excise dimers from the DNA of radioactively labeled unfractionated cell-free preparations.

Citing Articles

Domains in the XPA protein important in its role as a processivity factor.

L Bartels C, Lambert M Biochem Biophys Res Commun. 2007; 356(1):219-25.

PMID: 17349973 PMC: 1936984. DOI: 10.1016/j.bbrc.2007.02.125.

Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae.

Wilcox D, Prakash L J Bacteriol. 1981; 148(2):618-23.

PMID: 7028721 PMC: 216247. DOI: 10.1128/jb.148.2.618-623.1981.

Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.

Yarosh D, Setlow R Mol Cell Biol. 1981; 1(3):237-44.

PMID: 6965098 PMC: 369667. DOI: 10.1128/mcb.1.3.237-244.1981.

Excision of pyrimidine dimers from nuclear deoxyribonucleic acid in ultraviolet-irradiated Dictyostelium discoideum.

Clark J, DEERING R Mol Cell Biol. 1981; 1(2):121-7.

PMID: 6965095 PMC: 369650. DOI: 10.1128/mcb.1.2.121-127.1981.

Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.

Thompson L, Busch D, Brookman K, Mooney C, Glaser D Proc Natl Acad Sci U S A. 1981; 78(6):3734-7.

PMID: 6943579 PMC: 319646. DOI: 10.1073/pnas.78.6.3734.

References

Keijzer W, BOOTSMA D . Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization. Nat New Biol. 1972; 238(81):80-3. DOI: 10.1038/newbio238080a0. View

Kraemer K, ROBBINS J, Keijzer W, Barrett S, Petinga R, BOOTSMA D . Five complementation groups in xeroderma pigmentosum. Mutat Res. 1975; 33(2-3):327-40. DOI: 10.1016/0027-5107(75)90208-0. View

Bacchetti S, van der Plas A, VELDHUISEN G . A UV-specific endonucleolytic activity present in human cell extracts. Biochem Biophys Res Commun. 1972; 48(3):662-9. DOI: 10.1016/0006-291x(72)90399-3. View

Kraemer K, Coon H, Petinga R, Barrett S, Rahe A, ROBBINS J . Genetic heterogeneity in xeroderma pigmentosum: complementation groups and their relationship to DNA repair rates. Proc Natl Acad Sci U S A. 1975; 72(1):59-63. PMC: 432240. DOI: 10.1073/pnas.72.1.59. View

Tomura T, VAN LANCKER J . The effect of a mammalian repair endonuclease on x-irradiated DNA. Biochim Biophys Acta. 1975; 402(3):343-50. DOI: 10.1016/0005-2787(75)90270-1. View

Bacchetti S, Benne R . Purification and characterization of an endonuclease from calf thymus acting on irradiated DNA. Biochim Biophys Acta. 1975; 390(3):285-97. DOI: 10.1016/0005-2787(75)90349-4. View

Tanaka K, Sekiguchi M, Okada Y . Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus). Proc Natl Acad Sci U S A. 1975; 72(10):4071-5. PMC: 433140. DOI: 10.1073/pnas.72.10.4071. View

VAN LANCKER J, Tomura T . Purification and some properties of a mammalian repair endonuclease. Biochim Biophys Acta. 1974; 353(1):99-114. DOI: 10.1016/0005-2787(74)90101-4. View

Fornace Jr A, Kohn K, KANN Jr H . DNA single-strand breaks during repair of UV damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum. Proc Natl Acad Sci U S A. 1976; 73(1):39-43. PMC: 335834. DOI: 10.1073/pnas.73.1.39. View

10.

ROBBINS J, Kraemer K, LUTZNER M, Festoff B, Coon H . Xeroderma pigmentosum. An inherited diseases with sun sensitivity, multiple cutaneous neoplasms, and abnormal DNA repair. Ann Intern Med. 1974; 80(2):221-48. DOI: 10.7326/0003-4819-80-2-221. View

11.

Schneider E, Stanbridge E, Epstein C . Incorporation of 3H-uridine and 3H-uracil into RNA: a simple technique for the detection of mycoplasma contamination of cultured cells. Exp Cell Res. 1974; 84(1):311-8. DOI: 10.1016/0014-4827(74)90411-x. View

12.

Paterson M, Lohman P, Sluyter M . Use of UV endonuclease from Micrococcus luteus to monitor the progress of DNA repair in UV-irradiated human cells. Mutat Res. 1973; 19(2):245-56. DOI: 10.1016/0027-5107(73)90083-3. View

13.

Kleijer W, Sluyter M, Keijzer W . Repair replication in heterokaryons deprived from different repair-deficient xeroderma pigmentosum strains. Mutat Res. 1973; 19(2):237-43. DOI: 10.1016/0027-5107(73)90082-1. View

14.

Brent T . A human endonuclease activity for gamma-irradiated DNA. Biophys J. 1973; 13(4):399-401. PMC: 1484298. DOI: 10.1016/S0006-3495(73)85993-4. View

15.

Friedberg E, King J . Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease. J Bacteriol. 1971; 106(2):500-7. PMC: 285122. DOI: 10.1128/jb.106.2.500-507.1971. View

16.

Brent T . Partial purification of endonuclease activity from human lymphoblasts. Separation of activities for depurinated DNA and DNA irradiated with ultraviolet light. Biochim Biophys Acta. 1975; 407(2):191-9. DOI: 10.1016/0005-2787(75)90284-1. View

17.

Schneider E, Stanbridge E . Comparison of methods for the detection of Mycoplasmal contamination of cell cultures: a review. In Vitro. 1975; 11(1):20-34. DOI: 10.1007/BF02615318. View

18.

Cleaver J, BOOTSMA D . Xeroderma pigmentosum: biochemical and genetic characteristics. Annu Rev Genet. 1975; 9:19-38. DOI: 10.1146/annurev.ge.09.120175.000315. View

19.

Forger 3rd J, Choie D, Friedberg E . Non-histone chromosomal proteins of chemically transformed neoplastic cells in tissue culture. Cancer Res. 1976; 36(1):258-62. View

20.

Duker N, Teebor G . Different ultraviolet DNA endonuclease activity in human cells. Nature. 1975; 255(5503):82-4. DOI: 10.1038/255082a0. View