Repair of UV-induced Lesions in Xenopus Laevis Oocytes

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1987 Dec 1

PMID 2830488

Citations 15

Authors

R J Legerski

J E Penkala

C A Peterson

D A Wright

Affiliations

Soon will be listed here.

Abstract

We characterized a DNA repair system in frog oocytes by comicroinjection of UV-irradiated pBR322 DNA and radiolabeled nucleotides. Repair synthesis was monitored by incorporation of label into recovered pBR322 DNA and by a novel method in which the removal of UV photoproducts was determined from the shift of DNA topoisomers that occurs during gel electrophoresis upon repair of these lesions. We investigated the effects of several drugs in the oocyte system and found that although novobiocin, an inhibitor of topoisomerase II, was an effective inhibitor of repair, VM-26, another inhibitor of topoisomerase II, was not. In addition, the topoisomerase I inhibitor camptothecin had no effect on repair in this system. Finally, circular DNA (either supercoiled or nicked circular) was repaired at least 50 times more rapidly than linear DNA.

Citing Articles

Remarkable Enhancement of Nucleotide Excision Repair of a Bulky Guanine Lesion in a Covalently Closed Circular DNA Plasmid Relative to the Same Linearized Plasmid.

Kolbanovskiy M, Aharonoff A, Sales A, Geacintov N, Shafirovich V Biochemistry. 2020; 59(31):2842-2848.

PMID: 32786887 PMC: 7444659. DOI: 10.1021/acs.biochem.0c00441.

Processing of targeted psoralen cross-links in Xenopus oocytes.

Segal D, Faruqi A, Glazer P, Carroll D Mol Cell Biol. 1997; 17(11):6645-52.

PMID: 9343428 PMC: 232518. DOI: 10.1128/MCB.17.11.6645.

Assembly of DNA polymerase delta and epsilon holoenzymes depends on the geometry of the DNA template.

Podust L, Podust V, Floth C, Hubscher U Nucleic Acids Res. 1994; 22(15):2970-5.

PMID: 7915029 PMC: 310263. DOI: 10.1093/nar/22.15.2970.

Repair of heteroduplex DNA in Xenopus laevis oocytes.

Lehman C, Trautman J, Carroll D Genetics. 1994; 138(2):459-70.

PMID: 7828827 PMC: 1206162. DOI: 10.1093/genetics/138.2.459.

Repair of abasic sites by mammalian cell extracts.

Frosina G, Fortini P, Rossi O, Carrozzino F, Abbondandolo A, Dogliotti E Biochem J. 1994; 304 ( Pt 3):699-705.

PMID: 7818470 PMC: 1137391. DOI: 10.1042/bj3040699.

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