» Articles » PMID: 10588945

Structure and Functions of Human Oxysterol 7alpha-hydroxylase CDNAs and Gene CYP7B1

Overview
Journal J Lipid Res
Publisher Elsevier
Specialty Biochemistry
Date 1999 Dec 10
PMID 10588945
Citations 32
Authors
Affiliations
Soon will be listed here.
Abstract

Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA encodes a polypeptide of 506 amino acid residues that shares 40% sequence identity to human cholesterol 7alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in the conversion of cholesterol to bile acids in the liver. In contrast to the liver-specific expression of CYP7A1, CYP7B1 mRNA transcripts were detected in human tissues involved in steroid genesis (brain, testes, ovary, and prostate) and in bile acid synthesis (liver) and reabsorption (colon, kidney, and small intestine). The human oxysterol 7alpha-hydroxylase transiently expressed in 293/T cells was able to catalyze 7alpha-hydroxylation of 27-hydroxycholesterol and dehydroepiandrosterone (DHEA). The human CYP7A1 and CYP7B1 both contain six exons and five introns. However, CYP7B1 spans at least 65 kb of the genome and is about 6-fold longer than CYP7A1. The transcription start site (+1) was localized 204 bp upstream of the initiation codon. No TATA box-like sequence was found near the transcription start site. Transient transfection assays of CYP7B1 promoter/luciferase reporter constructs in HepG2 cells revealed that the promoter was highly active. The 5' upstream region from nt -83 to +189 is the core promoter of the gene.

Citing Articles

Association of CYP7B1 expression with the prognosis of endometrial cancer: a retrospective study.

Lu X, Huang T, Chen C, Zhang J, Fu X, Cheng B World J Surg Oncol. 2024; 22(1):251.

PMID: 39289693 PMC: 11406946. DOI: 10.1186/s12957-024-03504-4.


Discovery biomarker to optimize obeticholic acid treatment for non-alcoholic fatty liver disease.

Lee S, Won Jun D, Laurel Yoon E, Oh J, Roh Y, Lee E Biol Direct. 2023; 18(1):50.

PMID: 37626369 PMC: 10463927. DOI: 10.1186/s13062-023-00407-4.


SNPs in cytochrome P450 genes decide on the fate of individuals with genetic predisposition to Parkinson's disease.

Petkova-Kirova P, Baas S, Wagenpfeil G, Hartz P, Unger M, Bernhardt R Front Pharmacol. 2023; 14:1244516.

PMID: 37601072 PMC: 10436510. DOI: 10.3389/fphar.2023.1244516.


Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.

Yu L, Xu L, Chu H, Peng J, Sacharidou A, Hsieh H Nat Commun. 2023; 14(1):4101.

PMID: 37491347 PMC: 10368733. DOI: 10.1038/s41467-023-39586-z.


Mitochondrial Cholesterol Metabolites in a Bile Acid Synthetic Pathway Drive Nonalcoholic Fatty Liver Disease: A Revised "Two-Hit" Hypothesis.

Kakiyama G, Rodriguez-Agudo D, Pandak W Cells. 2023; 12(10).

PMID: 37408268 PMC: 10217489. DOI: 10.3390/cells12101434.