RIBP, a Novel Rlk/Txk- and Itk-binding Adaptor Protein That Regulates T Cell Activation

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1999 Dec 10

PMID 10587356

Citations 29

Authors

K Rajagopal

C L Sommers

D C Decker

E O Mitchell

U Korthauer

A I Sperling

C A Kozak

P E Love

J A Bluestone

Affiliations

Soon will be listed here.

Abstract

A novel T cell-specific adaptor protein, RIBP, was identified based on its ability to bind Rlk/Txk in a yeast two-hybrid screen of a mouse T cell lymphoma library. RIBP was also found to interact with a related member of the Tec family of tyrosine kinases, Itk. Expression of RIBP is restricted to T and natural killer cells and is upregulated substantially after T cell activation. RIBP-disrupted knockout mice displayed apparently normal T cell development. However, proliferation of RIBP-deficient T cells in response to T cell receptor (TCR)-mediated activation was significantly impaired. Furthermore, these activated T cells were defective in the production of interleukin (IL)-2 and interferon gamma, but not IL-4. These data suggest that RIBP plays an important role in TCR-mediated signal transduction pathways and that its binding to Itk and Rlk/Txk may regulate T cell differentiation.

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