Heat-labile B-cell Mitogen Obtained from Listeria Monocytogenes

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1978 Dec 1

PMID 103837

Citations 8

Authors

R J Kearns

D J Hinrichs

Affiliations

Soon will be listed here.

Abstract

A water-soluble extract of Listeria monocytogenes strain 10403 acts as a mitogen on cultured mouse spleen lymphocytes. This mitogen induced a response six to nine times that of controls, as measured by [3H]thymidine incorporation. The mitogen extract was derived from washed bacterial cells which were mechanically disrupted with a French press. The extract was centrifuged at 105,000 X g and filtered through a 0.22-micrometer filter. Similar levels of lymphocyte stimulation were observed in lymphocyte cultures prepared from spleens of nude mice, indicating the effect of this mitogen on B-cells. The mitogenic property of this extract was destroyed by heating to 56 degrees C. This heat treatment does not destroy the antigens in the extract, which stimulate spleen cell cultures obtained from specifically immune mice. Similarly prepared extracts from Staphylococcus epidermidis and Salmonella typhimurium did not show similar levels of mitogenic activity. The mitogenic property of the L. monocytogenes extract was present in two strains of Listeria tested and was not associated with mouse virulence.

Citing Articles

Recovery from T cell depletion during murine listeriosis and effect on a T-dependent antibody response.

Chan Y, Cheers C Infect Immun. 1982; 38(2):694-8.

PMID: 6982870 PMC: 347794. DOI: 10.1128/iai.38.2.694-698.1982.

Mechanism of depletion of T lymphocytes from the spleen of mice infected with Listeria monocytogenes.

Chan Y, Cheers C Infect Immun. 1982; 38(2):686-93.

PMID: 6982869 PMC: 347793. DOI: 10.1128/iai.38.2.686-693.1982.

Adjuvant activity of purified peptidoglycan of Listeria monocytogenes in mice and guinea pigs.

Saiki I, Kamisango K, Tanio Y, Okumura H, Yamamura Y, Azuma I Infect Immun. 1982; 38(1):58-65.

PMID: 6815094 PMC: 347697. DOI: 10.1128/iai.38.1.58-65.1982.

Flow microfluorometry analysis of alterations in T-lymphocyte subsets during murine listeriosis.

Watson S, Redington T, Miller T, Bullock W Infect Immun. 1984; 45(2):372-7.

PMID: 6611310 PMC: 263232. DOI: 10.1128/iai.45.2.372-377.1984.

Effect of dimethyl sulfoxide on the in vitro and in vivo bactericidal activity of human and mouse neutrophils and mononuclear phagocytes.

Czuprynski C, Henson P, Campbell P Inflammation. 1984; 8(2):181-91.

PMID: 6430803 DOI: 10.1007/BF00916093.

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