Ultrastructural Features of Chromatin Nu Bodies

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1976 Mar 1

PMID 1035912

Citations 13

Authors

A L Olins

M B Senior

D E Olins

Affiliations

Soon will be listed here.

Abstract

Spread chromatin fibers and isolated chromatin fragments prepared from chicken erythrocyte nuclei were stained with dilute aqueous uranyl acetate. High-resolution electron micrographs reveal two new morphological features exhibited by many of the chromatin nu bodies: (a) lateral association of the nu body with the connecting strand, and (b) a centrally stained spot approximately 15 A wide, possibly corresponding to a hole or crevice within the nu body.

Citing Articles

ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.

Ou H, Phan S, Deerinck T, Inagaki A, Ellisman M, OShea C Nat Protoc. 2024; .

PMID: 39613943 DOI: 10.1038/s41596-024-01071-2.

Interactions With Histone H3 & Tools to Study Them.

Scott W, Campos E Front Cell Dev Biol. 2020; 8:701.

PMID: 32850821 PMC: 7411163. DOI: 10.3389/fcell.2020.00701.

ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells.

Ou H, Phan S, Deerinck T, Thor A, Ellisman M, OShea C Science. 2017; 357(6349).

PMID: 28751582 PMC: 5646685. DOI: 10.1126/science.aag0025.

Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells.

Dalal Y, Wang H, Lindsay S, Henikoff S PLoS Biol. 2007; 5(8):e218.

PMID: 17676993 PMC: 1933458. DOI: 10.1371/journal.pbio.0050218.

Electron microscopy of the nucleocapsid from disrupted Moloney murine leukemia virus and of associated type VI collagen-like filaments.

Pager J, Coulaud D, Delain E J Virol. 1994; 68(1):223-32.

PMID: 8254732 PMC: 236281. DOI: 10.1128/JVI.68.1.223-232.1994.

References

Zobel C, Beer M . Electron stains. I. Chemical studies on the interaction of DNA with uranyl salts. J Biophys Biochem Cytol. 1961; 10:335-46. PMC: 2225082. DOI: 10.1083/jcb.10.3.335. View

Olins A, Olins D . Spheroid chromatin units (v bodies). Science. 1974; 183(4122):330-2. DOI: 10.1126/science.183.4122.330. View

Olins D, WRIGHT E . Glutaraldehyde fixation of isolated eucaryotic nuclei. Evidence for histone-histone proximity. J Cell Biol. 1973; 59(2 Pt 1):304-17. PMC: 2109091. DOI: 10.1083/jcb.59.2.304. View

Weintraub H, Van Lente F . Dissection of chromosome structure with trypsin and nucleases. Proc Natl Acad Sci U S A. 1974; 71(10):4249-53. PMC: 434369. DOI: 10.1073/pnas.71.10.4249. View

DAnna Jr J, Isenberg I . A histone cross-complexing pattern. Biochemistry. 1974; 13(24):4992-7. DOI: 10.1021/bi00721a019. View

van Bruggen E, Arnberg A, VAN Holde K, Sahasrabuddhe C, Shaw B . Electron microscopy of chromatin subunit particles. Biochem Biophys Res Commun. 1974; 60(4):1365-70. DOI: 10.1016/0006-291x(74)90348-9. View

Noll M . Subunit structure of chromatin. Nature. 1974; 251(5472):249-51. DOI: 10.1038/251249a0. View

DAnna Jr J, Isenberg I . Interactions of histone LAK (f2a2) with histones KAS (f2b) and GRK (f2a1). Biochemistry. 1974; 13(10):2098-104. DOI: 10.1021/bi00707a016. View

Kornberg R . Chromatin structure: a repeating unit of histones and DNA. Science. 1974; 184(4139):868-71. DOI: 10.1126/science.184.4139.868. View

10.

Kornberg R, Thomas J . Chromatin structure; oligomers of the histones. Science. 1974; 184(4139):865-8. DOI: 10.1126/science.184.4139.865. View

11.

Kelley R . Isolation of a histone IIb1-IIb2 complex. Biochem Biophys Res Commun. 1973; 54(4):1588-94. DOI: 10.1016/0006-291x(73)91168-6. View

12.

Rill R, VAN Holde K . Properties of nuclease-resistant fragments of calf thymus chromatin. J Biol Chem. 1973; 248(3):1080-3. View

13.

DAnna Jr J, Isenberg I . A complex of histones IIb2 and IV. Biochemistry. 1973; 12(6):1035-43. DOI: 10.1021/bi00730a003. View

14.

Hewish D, Burgoyne L . Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973; 52(2):504-10. DOI: 10.1016/0006-291x(73)90740-7. View

15.

Skandrani E, MIZON J, Sautiere P, BISERTE G . [Study of the F2b fraction of calf thymus histones]. Biochimie. 1972; 54(10):1267-72. DOI: 10.1016/s0300-9084(72)80067-1. View

16.

Pardon J, Wilkins M . A super-coil model for nucleohistone. J Mol Biol. 1972; 68(1):115-24. DOI: 10.1016/0022-2836(72)90267-7. View

17.

Van Lente F, Jackson J, Weintraub H . Identification of specific crosslinked histones after treatment of chromatin with formaldehyde. Cell. 1975; 5(1):45-50. DOI: 10.1016/0092-8674(75)90090-2. View

18.

Oosterhof D, Hozier J, Rill R . Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils. Proc Natl Acad Sci U S A. 1975; 72(2):633-7. PMC: 432368. DOI: 10.1073/pnas.72.2.633. View

19.

Weintraub H . Release of discrete subunits after nuclease and trypsin digestion of chromatin. Proc Natl Acad Sci U S A. 1975; 72(3):1212-6. PMC: 432498. DOI: 10.1073/pnas.72.3.1212. View

20.

Olins A, Carlson R, Olins D . Visualization of chromatin substructure: upsilon bodies. J Cell Biol. 1975; 64(3):528-37. PMC: 2109536. DOI: 10.1083/jcb.64.3.528. View