Identification of Specific Crosslinked Histones After Treatment of Chromatin with Formaldehyde

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 1975 May 1

PMID 805667

Citations 18

Authors

F Van Lente

J F Jackson

H Weintraub

Affiliations

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Abstract

Treatment of chromatin or nuclei with formaldehyde or glutaraldehyde results in the appearance of crosslinked histones as analyzed on SDS gels. The identity of two major crosslinked products was determined by iodinating the product and analzying the tryptic peptides. The results show that one product is a dimer of histones IIb2 and IIb1 and another product is a dimer of histones IIb2 and IV. These species represent no more than 15% of the total cellular histone.

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